Evidence map›Paper›PMID 37292669›Full record

ArticlebioRxiv : the preprint server for biology2023

Creating Optimal Conditions for OPA1 Isoforms by Western Blot in Skeletal Muscle Cells and Tissue.

Dominique C Stephens, Margaret Mungai, Amber Crabtree, Heather K Beasley, Edgar Garza-Lopez, Kit Neikirk, Serif Bacevac, Larry Vang, Zer Vue, Neng Vue and 9 more

Open access · greenAbstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

19 authors at 7 institutions in 1 country.

Dominique C StephensDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Margaret MungaiDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Amber CrabtreeDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Heather K BeasleyDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Edgar Garza-LopezDepartment of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA.
Kit NeikirkDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Serif BacevacCentral Microscopy Research Facility, University of Iowa, Iowa City, IA, 52242, USA.
Larry VangDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Zer VueDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Neng VueDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Andrea G MarshallDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Kyrin TurnerDepartment of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA.
Jianqiang ShaoCentral Microscopy Research Facility, University of Iowa, Iowa City, IA, 52242, USA.
Sandra MurrayDepartment of Cell Biology, College of Medicine, University of Pittsburgh, Pittsburgh, PA, 15260, USA.
Jennifer A GaddyDivision of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, TN, 37232, USA.
Celestine WanjallaVanderbilt University Medical Center: Department of Medicine, Division of Infectious Disease, Nashville, TN, USA.
Jamaine DavisDepartment of Biochemistry and Cancer Biology. Meharry Medical College, Nashville, TN, 37208, USA.
Steven DamoDepartment of Life and Physical Sciences, Fisk University, Nashville, TN, 37232, USA.
Antentor O HintonDepartment of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA.
Vanderbilt University · USUniversity of Iowa · USFisk University · USMeharry Medical College · USUnited States Department of Veterans Affairs · USUniversity of Pittsburgh · USVanderbilt University Medical Center · US

Funding

VANDERBILT UNIVERSITY CTSA FOR PEDIATRIC RESEARCHUL1RR024975 · NCRR · VANDERBILT UNIVERSITY · PI BERNARD, GORDON RAPHAEL · 2007 to 2011
$45.7M
The Vanderbilt Institute for Clinical and Translational Research (VICTR)UL1TR000445 · NCATS · VANDERBILT UNIVERSITY MEDICAL CENTER · PI BERNARD, GORDON RAPHAEL · 2012 to 2016
$41.4M
Vanderbilt Diabetes Research CenterP30DK020593 · NIDDK · VANDERBILT UNIVERSITY MEDICAL CENTER · PI ALVIN C POWERS · 2012 to 2026
$29.3M
MULTIDISCIPLINARY TRAINING IN MOLECULAR ENDOCRINOLOGYT32DK007563 · NIDDK · VANDERBILT UNIVERSITY · PI Richard M O'Brien · 1988 to 2026
$15.9M
Training In Cardiovascular Physiology & PharmacologyT32HL007444 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Ju Chen, Robert Scott Ross · 1985 to 2026
$11.1M
PRIDE: Functional and Translational Genomics of Blood DisordersR25HL106365 · NHLBI · AUGUSTA UNIVERSITY · PI PACE, BETTY SUE · 2010 to 2024
$5.2M
Integrated Training in Engineering and DiabetesT32DK101003 · NIDDK · VANDERBILT UNIVERSITY · PI Jamey D. Young · 2014 to 2026
$3.9M
UCSD PRIDE Faculty Development Program in Cardiovascular SciencesR25HL145817 · NHLBI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Robert Scott Ross, Joann Trejo · 2019 to 2026
$3.3M
Determining the contribution of zinc deficiency to perinatal Group B Streptococcus infectionsR01HD090061 · NICHD · VANDERBILT UNIVERSITY MEDICAL CENTER · PI ARONOFF, DAVID M, GADDY, JENNIFER A · 2017 to 2021
$2.5M
BLRD VA I01 BX005352NCATS NIH HHS UL1 TR000445NCRR NIH HHS UL1 RR024975NHLBI NIH HHS R25 HL106365NHLBI NIH HHS R25 HL145817NHLBI NIH HHS T32 HL007444NICHD NIH HHS R01 HD090061NIDDK NIH HHS P30 DK020593NIDDK NIH HHS T32 DK007563NIDDK NIH HHS T32 DK101003
6 · The paper itself

Abstract

OPA1 is a dynamin-related GTPase that modulates various mitochondrial functions and is involved in mitochondrial morphology. There are eight different isoforms of OPA1 in humans and five different isoforms in mice that are expressed as short or long-form isoforms. These isoforms contribute to OPA1's ability to control mitochondrial functions. However, isolating OPA1 all long and short isoforms through western blot has been a difficult task. To address this issue, we outline an optimized western blot protocol to isolate 5 different isoforms of OPA1 on the basis of different antibodies. This protocol can be used to study changes in mitochondrial structure and function.

Indexed as

isoformsisolationMitochondriaMuscle TissueOptic atrophy-1 (OPA1)Western Blot

Identifiers

PMID37292669
PMCPMC10245902
OpenAlexW4377245759

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.