ArticleNature structural & molecular biology2023
RNA polymerase II depletion from the inactive X chromosome territory is not mediated by physical compartmentalization.
Article in Nature structural & molecular biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 37 citations in OpenAlex.
- Lyonization of X-chromosome: New insights.Journal of genetics · 2026Review
- Illuminating biomolecular assemblies in gene regulation.Biophysical reviews · 2025Review
- Xist condensates: perspectives for therapeutic intervention.Genome biology · 2025Review
- Role of the SAF-A/HNRNPU SAP domain in X chromosome inactivation, nuclear dynamics, transcription, splicing, and cell proliferation.PLoS genetics · 2025Article
- Tri-omic single-cell mapping of the 3D epigenome and transcriptome in whole mouse brains throughout the lifespan.Nature methods · 2025Article
- CRL3Molecular cell · 2024Article
- Article
- Compensation of gene dosage on the mammalian X.Development (Cambridge, England) · 2024Review
- A critical role for X-chromosome architecture in mammalian X-chromosome dosage compensation.Current opinion in genetics & development · 2024Review
- Dynamic microenvironments shape nuclear organization and gene expression.Current opinion in genetics & development · 2024Review
- RNA: The Unsuspected Conductor in the Orchestra of Macromolecular Crowding.Chemical reviews · 2024Review
- Comparative Bioinformatic Analysis of the Proteomes of Rabbit and Human Sex Chromosomes.Animals : an open access journal from MDPI · 2024Article
- Review
- Transcription and replication meet the silent X chromosome territory.Nature structural & molecular biology · 2023Article
- Single-molecule tracking (SMT): a window into live-cell transcription biochemistry.Biochemical Society transactions · 2023Article
- CIZ1 in Xist seeded assemblies at the inactive X chromosome.Frontiers in cell and developmental biology · 2023Review
- Detecting and quantifying liquid-liquid phase separation in living cells by model-free calibrated half-bleaching.Nature communications · 2022Article
- Chromatin accessibility: methods, mechanisms, and biological insights.Nucleus (Austin, Tex.) · 2022Review
- Simultaneous mapping of 3D structure and nascent RNAs argues against nuclear compartments that preclude transcription.Cell reports · 2022Article
- The Molecular and Nuclear Dynamics of X-Chromosome Inactivation.Cold Spring Harbor perspectives in biology · 2022Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 4 institutions in 3 countries.
Funding
Abstract
Subnuclear compartmentalization has been proposed to play an important role in gene regulation by segregating active and inactive parts of the genome in distinct physical and biochemical environments. During X chromosome inactivation (XCI), the noncoding Xist RNA coats the X chromosome, triggers gene silencing and forms a dense body of heterochromatin from which the transcription machinery appears to be excluded. Phase separation has been proposed to be involved in XCI, and might explain the exclusion of the transcription machinery by preventing its diffusion into the Xist-coated territory. Here, using quantitative fluorescence microscopy and single-particle tracking, we show that RNA polymerase II (RNAPII) freely accesses the Xist territory during the initiation of XCI. Instead, the apparent depletion of RNAPII is due to the loss of its chromatin stably bound fraction. These findings indicate that initial exclusion of RNAPII from the inactive X reflects the absence of actively transcribing RNAPII, rather than a consequence of putative physical compartmentalization of the inactive X heterochromatin domain.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.