Evidence map›Paper›PMID 37291398›Full record

ArticleNature plants2023

Grafting Rhodobacter sphaeroides with red algae Rubisco to accelerate catalysis and plant growth.

Yu Zhou, Laura H Gunn, Rosemary Birch, Inger Andersson, Spencer M Whitney

Abstract read
PubMed Publisher
In one paragraph

Article in Nature plants, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
3.7field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 25 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Review
  9. Review
  10. Review
  11. Rubisco is evolving for improved catalytic efficiency and COProceedings of the National Academy of Sciences of the United States of America · 2024
    Article
  12. Engineering Rubisco to enhance COSynthetic and systems biotechnology · 2024
    Review
  13. Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 4 countries.

Yu Zhou *Plant Science Division, Research School of Biology, The Australian National University, Canberra, Australian Capital Territory, Australia.ORCID http://orcid.org/0000-0002-6828-0120
Laura H Gunn *Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
Rosemary BirchPlant Science Division, Research School of Biology, The Australian National University, Canberra, Australian Capital Territory, Australia.
Inger AnderssonLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden.
Spencer M WhitneyPlant Science Division, Research School of Biology, The Australian National University, Canberra, Australian Capital Territory, Australia. spencer.whitney@anu.edu.au.ORCID http://orcid.org/0000-0003-2954-2359
Australian National University · AUUppsala University · SE

Funding

Department of Education and Training | Australian Research Council (ARC) CE140100015EC | European Regional Development Fund (Europski Fond za Regionalni Razvoj) CZ.02.1.01/0.0/0.0/15_003/0000447
6 · The paper itself

Abstract

Improving the carboxylation properties of Rubisco has primarily arisen from unforeseen amino acid substitutions remote from the catalytic site. The unpredictability has frustrated rational design efforts to enhance plant Rubisco towards the prized growth-enhancing carboxylation properties of red algae Griffithsia monilis GmRubisco. To address this, we determined the crystal structure of GmRubisco to 1.7 Å. Three structurally divergent domains were identified relative to the red-type bacterial Rhodobacter sphaeroides RsRubisco that, unlike GmRubisco, are expressed in Escherichia coli and plants. Kinetic comparison of 11 RsRubisco chimaeras revealed that incorporating C329A and A332V substitutions from GmRubisco Loop 6 (corresponding to plant residues 328 and 331) into RsRubisco increased the carboxylation rate (k

Indexed as

Rhodobacter sphaeroidesRhodophytaCatalysisPhotosynthesisPlantsRibulose-Bisphosphate CarboxylaseRibulose-Bisphosphate Carboxylase

Identifiers

PMID37291398
OpenAlexW4379878707

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.