ArticleNature chemical biology2023
A general method for the development of multicolor biosensors with large dynamic ranges.
Article in Nature chemical biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed.
- Solute carrier membrane transporters: emerging targets in CNS disorders.Nature reviews. Drug discovery · 2026Review
- Genetically encoded tools for tracking metabolites in live cells.Biochemical Society transactions · 2026Review
- Synthesis, Characterization, and Fluorescence Application of Indole-Based Isofunctional NADChembiochem : a European journal of chemical biology · 2026Article
- Modular Input-Output Biosensor Design UsingACS sensors · 2026Article
- Sensing and Reprogramming Surface Receptor Activation With Synthetic Transcriptional Circuits.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Vision of Life's Code: Molecular Probe Nanoarchitectonics for Deep RNA/DNA Illumination.ACS nano · 2026Review
- Chemogenetic detection and quantitation of HNature protocols · 2026Review
- Protein engineering: status report.Protein engineering, design & selection : PEDS · 2026Review
- A Photoswitchable HaloTag for Spatiotemporal Control of Fluorescence in Living Cells.Angewandte Chemie (International ed. in English) · 2025Article
- Synthetic fluorophores for live-cell fluorescence microscopy and biosensing.Nature chemical biology · 2025Review
- Mitochondrial NADScience advances · 2025Article
- ATP Metabolism of Astrocytes: Consumption, Regeneration and Restoration.Neurochemical research · 2025Review
- Molecular recording of cellular protein kinase activity with chemical labeling.Nature chemical biology · 2025Article
- An Improved Ratiometric FRET Biosensor with Higher Affinity for Extracellular ATP.Sensors (Basel, Switzerland) · 2025Article
- Three-dimensional traction technology and its application in mechanomedicine.Mechanobiology in medicine · 2025Review
- State of the art indicators for imaging purinergic dynamics in vitro and in vivo.Purinergic signalling · 2025Review
- Recent advances in spying on cell signaling with fluorescent biosensors.Current opinion in cell biology · 2025Review
- Review
- Ratio-based indicators for cytosolic CaProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Engineering modular and tunable single-molecule sensors by decoupling sensing from signal output.Nature nanotechnology · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescent biosensors enable the study of cell physiology with spatiotemporal resolution; yet, most biosensors suffer from relatively low dynamic ranges. Here, we introduce a family of designed Förster resonance energy transfer (FRET) pairs with near-quantitative FRET efficiencies based on the reversible interaction of fluorescent proteins with a fluorescently labeled HaloTag. These FRET pairs enabled the straightforward design of biosensors for calcium, ATP and NAD
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