Evidence map›Paper›PMID 37290935›Full record

ArticleGenome research2023

Genome-scale targeted mutagenesis in

Jianjie He, Kai Zhang, Shuxiang Yan, Mi Tang, Weixian Zhou, Yongtai Yin, Kang Chen, Chunyu Zhang, Maoteng Li

Abstract read
In one paragraph

Article in Genome research, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Review
  6. Article
  7. Targeted mutation ofHorticulture research · 2025
    Article
  8. Review
  9. Article
  10. Article
  11. CRISPR-Cas systems and applications for crop bioengineering.Frontiers in bioengineering and biotechnology · 2024
    Review
  12. Transcriptome Shock in Developing Embryos of aInternational journal of molecular sciences · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jianjie He *Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Kai Zhang *Department of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Shuxiang YanDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Mi TangDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Weixian ZhouDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Yongtai YinDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Kang ChenDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Chunyu ZhangNational Key Laboratory of Crop Genetic Improvement and College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China zhchy@mail.hzau.edu.cn limaoteng426@hust.edu.cn.
Maoteng LiDepartment of Biotechnology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China; zhchy@mail.hzau.edu.cn limaoteng426@hust.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The recently constructed mutant libraries of diploid crops by the CRISPR-Cas9 system have provided abundant resources for functional genomics and crop breeding. However, because of the genome complexity, it is a big challenge to accomplish large-scale targeted mutagenesis in polyploid plants. Here, we demonstrate the feasibility of using a pooled CRISPR library to achieve genome-scale targeted editing in an allotetraploid crop of

Indexed as

Brassica napusCRISPR-Cas SystemsGene EditingMutagenesisPlant BreedingPlants, Genetically ModifiedPolyploidy

Identifiers

PMID37290935
PMCPMC10317123

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.