Evidence map›Paper›PMID 37289058›Full record

ArticleMicrobiology spectrum2023

Diploid Nuclei Occur throughout the Life Cycles of Pucciniales Fungi.

Pedro Talhinhas, Rita Carvalho, Sílvia Tavares, Teresa Ribeiro, Helena Azinheira, Ana Paula Ramos, Maria do Céu Silva, Marta Monteiro, João Loureiro, Leonor Morais-Cecílio

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Pedro TalhinhasLEAF-Linking Landscape, Environment, Agriculture and Food Research Centre and Terra Associated Laboratory, Instituto Superior de Agronomia, Universidade de Lisboa, Lisbon, Portugal.ORCID 0000-0001-6694-7502
Rita CarvalhoLEAF-Linking Landscape, Environment, Agriculture and Food Research Centre and Terra Associated Laboratory, Instituto Superior de Agronomia, Universidade de Lisboa, Lisbon, Portugal.
Sílvia TavaresSection for Plant and Soil Science, Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg, Copenhagen, Denmark.
Teresa RibeiroLEAF-Linking Landscape, Environment, Agriculture and Food Research Centre and Terra Associated Laboratory, Instituto Superior de Agronomia, Universidade de Lisboa, Lisbon, Portugal.
Helena AzinheiraLEAF-Linking Landscape, Environment, Agriculture and Food Research Centre and Terra Associated Laboratory, Instituto Superior de Agronomia, Universidade de Lisboa, Lisbon, Portugal.
Ana Paula RamosLEAF-Linking Landscape, Environment, Agriculture and Food Research Centre and Terra Associated Laboratory, Instituto Superior de Agronomia, Universidade de Lisboa, Lisbon, Portugal.
Maria do Céu SilvaLEAF-Linking Landscape, Environment, Agriculture and Food Research Centre and Terra Associated Laboratory, Instituto Superior de Agronomia, Universidade de Lisboa, Lisbon, Portugal.
Marta MonteiroInstituto Gulbenkian de Ciência, Oeiras, Portugal.
João LoureiroCFE-Centre for Functional Ecology and Terra Associated Laboratory, Departamento de Ciências da Vida, Universidade de Coimbra, Coimbra, Portugal.
Leonor Morais-CecílioLEAF-Linking Landscape, Environment, Agriculture and Food Research Centre and Terra Associated Laboratory, Instituto Superior de Agronomia, Universidade de Lisboa, Lisbon, Portugal.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Within Eukaryotes, fungi are the typical representatives of haplontic life cycles. Basidiomycota fungi are dikaryotic in extensive parts of their life cycle, but diploid nuclei are known to form only in basidia. Among Basidiomycota, the Pucciniales are notorious for presenting the most complex life cycles, with high host specialization, and for their expanded genomes. Using cytogenomic (flow cytometry and cell sorting on propidium iodide-stained nuclei) and cytogenetic (FISH with rDNA probe) approaches, we report the widespread occurrence of replicating haploid and diploid nuclei (i.e., 1C, 2C and a small proportion of 4C nuclei) in diverse life cycle stages (pycnial, aecial, uredinial, and telial) of all 35 Pucciniales species analyzed, but not in sister taxa. These results suggest that the Pucciniales life cycle is distinct from any cycle known, i.e., neither haplontic, diplontic nor haplodiplontic, corroborating patchy and disregarded previous evidence. However, the biological basis and significance of this phenomenon remain undisclosed.

Indexed as

BasidiomycotaDiploidyAnimalsFungiLife Cycle StagesMeiosisBasidiomycotadiploid nucleiFISHflow cytometryhaploid nucleinuclear cyclePuccinialesrust fungi

Identifiers

PMID37289058
PMCPMC10433954

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.