Evidence map›Paper›PMID 37288813›Full record

ArticleJournal of cell science2023

Direct fluorescent labeling of NF186 and NaV1.6 in living primary neurons using bioorthogonal click chemistry.

Nevena Stajković, Yuanyuan Liu, Aleksandra Arsić, Ning Meng, Hang Lyu, Nan Zhang, Dirk Grimm, Holger Lerche, Ivana Nikić-Spiegel

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cell science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.6field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 11 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Review
  5. Review
  6. Bioorthogonal Reactions in Bioimaging.Topics in current chemistry (Cham) · 2024
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Nevena StajkovićWerner Reichardt Centre for Integrative Neuroscience, University of Tübingen, 72076 Tübingen, Germany.ORCID 0000-0002-6661-7711
Yuanyuan LiuDepartment of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, University of Tübingen, 72076 Tübingen, Germany.ORCID 0000-0002-4273-821X
Aleksandra ArsićWerner Reichardt Centre for Integrative Neuroscience, University of Tübingen, 72076 Tübingen, Germany.ORCID 0000-0001-6248-8580
Ning MengVirus-Host Interaction Group, Department of Infectious Diseases/Virology, Medical Faculty, University of Heidelberg, Cluster of Excellence CellNetworks, BioQuant, 69120 Heidelberg, Germany.
Hang LyuGraduate Training Centre of Neuroscience, International Max Planck Research School, University of Tübingen, 72076 Tübingen, Germany.
Nan ZhangDepartment of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, University of Tübingen, 72076 Tübingen, Germany.
Dirk GrimmVirus-Host Interaction Group, Department of Infectious Diseases/Virology, Medical Faculty, University of Heidelberg, Cluster of Excellence CellNetworks, BioQuant, 69120 Heidelberg, Germany.ORCID 0000-0001-6227-5665
Holger LercheDepartment of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, University of Tübingen, 72076 Tübingen, Germany.ORCID 0000-0002-1783-8710
Ivana Nikić-SpiegelWerner Reichardt Centre for Integrative Neuroscience, University of Tübingen, 72076 Tübingen, Germany.ORCID 0000-0002-6666-3486
Hertie Institute for Clinical Brain Research · DEUniversity of Tübingen · DEHeidelberg University · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The axon initial segment (AIS) is a highly specialized neuronal compartment that regulates the generation of action potentials and maintenance of neuronal polarity. Live imaging of the AIS is challenging due to the limited number of suitable labeling methods. To overcome this limitation, we established a novel approach for live labeling of the AIS using unnatural amino acids (UAAs) and click chemistry. The small size of UAAs and the possibility of introducing them virtually anywhere into target proteins make this method particularly suitable for labeling of complex and spatially restricted proteins. Using this approach, we labeled two large AIS components, the 186 kDa isoform of neurofascin (NF186; encoded by Nfasc) and the 260 kDa voltage-gated Na+ channel (NaV1.6, encoded by Scn8a) in primary neurons and performed conventional and super-resolution microscopy. We also studied the localization of epilepsy-causing NaV1.6 variants with a loss-of-function effect. Finally, to improve the efficiency of UAA incorporation, we developed adeno-associated viral (AAV) vectors for click labeling in neurons, an achievement that could be transferred to more complex systems such as organotypic slice cultures, organoids, and animal models.

Indexed as

Axon Initial SegmentClick ChemistryAction PotentialsAmino AcidsAnimalsMiceNeuronsRatsAmino AcidsAxon initial segmentClick chemistryMicroscopyNeurofascinUnnatural amino acidVoltage-gated Na+ channel

Identifiers

PMID37288813
PMCPMC10323244
OpenAlexW4379768184

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.