Evidence map›Paper›PMID 37287826›Full record

ArticleBiophysics reports2022

Principles of fluorescence correlation spectroscopy applied to studies of biomolecular liquid-liquid phase separation.

Zhulou Wang, Huizhi Zhang, Lin Jian, Bo Ding, Keying Huang, Wolun Zhang, Qian Xiao, Shaohui Huang

Open access · diamondAbstract read
In one paragraph

Article in Biophysics reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 14 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Zhulou WangInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Huizhi ZhangInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Lin JianInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Bo DingLightEdge Technologies Limited, Zhongshan, Guangdong 528403, China.
Keying HuangLightEdge Technologies Limited, Zhongshan, Guangdong 528403, China.
Wolun ZhangLightEdge Technologies Limited, Zhongshan, Guangdong 528403, China.
Qian XiaoInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Shaohui HuangInstitute of Biophysics, Chinese Academy of Sciences, Beijing 100101, China.
Chinese Academy of Sciences · CNInstitute of Biophysics · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescence correlation spectroscopy (FCS) investigates the temporal relationship of fluctuating fluorescence signals reflecting underlying molecular processes occurring in a solution sample or a single live cell. This review article introduces the principles of two basic and most used FCS techniques: fluorescence auto-correlation spectroscopy (FACS) and fluorescence cross-correlation spectroscopy (FCCS). Combined, FACS and FCCS techniques can quantitatively analyze multiple properties of molecule or nanoparticle samples, including molar concentration, diffusion coefficient and hydrodynamic radius, homo- or hetero-interaction, fluorescence brightness,

Indexed as

Binding affinityDiffusion coefficientFluorescence correlation spectroscopyHydrodynamic radiusLiquid–liquid phase separation

Identifiers

PMID37287826
PMCPMC10195812
OpenAlexW4221012612

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.