ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2023
Development of the paper-based colorimetric sensor for simple and fast determination of quercetin in guava leaf extract.
Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
This present study aimed to develop a paper-based colorimetric sensor in the form of paper-microzone plates (PµZP), for simple and fast quercetin determination in guava leaf extract samples. Here, N-bromosuccinimide (NBS) solution was immobilized on the microzone as a sensing probe, where quercetin solution can be dropped on it to form red-purplish color adducts which can be seen by the naked eye or captured using a flatbed scanner. The color intensity of the microzone can be quantified against a blank solution and used as analytical data in scanometric assay. The sensor showed a response time of 8 min, a linear interval of 1-10 mM with a detection limit at 1.274 mM toward quercetin, and exhibited good reproducibility (RSD < 1%) and accuracy (98-99% recovery). The quercetin level of guava leaf extract determined by the PµZP-scanometric method was found comparable with that of the TLC-densitometric method, suggesting its use as an alternative method for quercetin analysis in the guava leaf extract.
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