ArticleResearch and practice in thrombosis and haemostasis2023
Preventing swarm detection in extracellular vesicle flow cytometry: a clinically applicable procedure.
Article in Research and practice in thrombosis and haemostasis, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Protocol for fluorescence-activated mitochondria sorting in endoplasmic reticulum-mitochondria contact reporter cells.STAR protocols · 2026Article
- Measurement of extracellular vesicles using flow cytometry: friend or foe?Research and practice in thrombosis and haemostasis · 2026Article
- The Dilution Paradox in Extracellular Vesicle Flow Cytometry.Journal of extracellular vesicles · 2026Article
- Resolving the dilution paradox to improve the interpretation of extracellular vesicle biomarker studies.Research and practice in thrombosis and haemostasis · 2026Article
- Flow Cytometric Detection of Biomarker Changes in CFDA-SE-Labelled Plasma Extracellular Vesicles Using a Rodent Pregnancy Model of Prenatal Diagnostics.Journal of extracellular biology · 2026Article
- Detection of plasma EV-associated TRAIL by nanoscale flow cytometry for liver metastasis prediction in PDAC.Advanced biotechnology · 2026Article
- The Squalene Oil-in-Water Nanoemulsion Vaccine Carrier AddaVax Potentiates TLR-Induced B-Cell Responses.Cytometry. Part A : the journal of the International Society for Analytical Cytology · 2026Article
- Particle size distribution and concentration of IntralipidJournal of biomedical optics · 2026Article
- Refractive index of milk fat globules and extracellular vesicles in human milk.Biophotonics discovery · 2026Article
- Calibration of Flow Cytometers Enables Reproducible Measurements of Extracellular Vesicle Concentrations and Reference Range Establishment.Journal of extracellular vesicles · 2025Article
- Identification of Ischemic Stroke Patients Based on Plasma Concentrations of Extracellular Vesicles.Translational stroke research · 2025Article
- Refining Flow Cytometry-based Sorting of Plasma-derived Extracellular Vesicles.Biological procedures online · 2025Article
- Targeting Oncofoetal Chondroitin Sulphate Allows Identification of Tumour-Derived Extracellular Vesicles.Journal of extracellular vesicles · 2025Article
- Nano-Flow Cytometry-Guided Discrimination and Separation of Human Cytomegalovirus Virions and Extracellular Vesicles.Journal of extracellular vesicles · 2025Article
- Spectral flow cytometry for detecting DNA cargo in malaria parasite-derived extracellular vesicles.The Journal of biological chemistry · 2025Article
- Approaches and Challenges in Characterizing the Molecular Content of Extracellular Vesicles for Biomarker Discovery.Biomolecules · 2024Review
- Article
- M1-derived extracellular vesicles polarize recipient macrophages into M2-like macrophages and alter skeletal muscle homeostasis in a hyper-glucose environment.Cell communication and signaling : CCS · 2024Article
- Lipoprotein apheresis affects the concentration of extracellular vesicles in patients with elevated lipoprotein (a).Scientific reports · 2024Article
- Poloxamer-188 as a wetting agent for microfluidic resistive pulse sensing measurements of extracellular vesicles.PloS one · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Flow cytometry is commonly used to detect cell-derived extracellular vesicles in body fluids such as blood plasma. However, continuous and simultaneous illumination of multiple particles at or below the detection limit may result in the detection of a single event. This phenomenon is called swarm detection and leads to incorrect particle concentration measurements. To prevent swarm detection, sample dilution is recommended. Since the concentration of particles differs between plasma samples, finding the optimal sample dilution requires dilution series of all samples, which is unfeasible in clinical routine. Objectives: Here we developed a practical procedure to find the optimal sample dilution of plasma for extracellular vesicle flow cytometry measurements in clinical research studies. Methods: Dilution series of 5 plasma samples were measured with flow cytometry (Apogee A60-Micro), triggered on side scatter. The total particle concentration between these plasma samples ranged from 2.5 × 10 Results: Swarm detection was absent in plasma samples when diluted ≥1.1 × 10 Conclusion: To prevent swarm detection in a series of clinical samples, the measurement count rate of a single diluted plasma sample can be used to determine the optimal dilution factor. For our samples, flow cytometer, and settings, the optimal dilution factor is ≥1.1 × 10
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.