Evidence map›Paper›PMID 37284166›Full record

ArticleFree neuropathology2022

Ex situ perfusion fixation for brain banking: a technical report.

Andrew T McKenzie, Emma Woodoff-Leith, Diana Dangoor, Alessandra Cervera, Hadley Walsh Ressler, Kristen Whitney, Kristen Dams-O'Connor, Zhuhao Wu, Elizabeth M C Hillman, Alan C Seifert and 1 more

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Free neuropathology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.

0numbers the graph read from it
0cells of the map it votes in
12citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

12 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
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  3. Cortical OProceedings of the National Academy of Sciences of the United States of America · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Andrew T McKenzieDepartment of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Emma Woodoff-LeithDepartment of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Diana DangoorDepartment of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Alessandra CerveraDepartment of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Hadley Walsh ResslerDepartment of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Kristen WhitneyDepartment of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Kristen Dams-O'ConnorDepartment of Rehabilitation and Human Performance, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Zhuhao WuDepartment of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Elizabeth M C HillmanLaboratory for Functional Optical Imaging, Departments of Biomedical Engineering and Radiology and the Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, New York, New York, USA.
Alan C SeifertBiomedical Engineering and Imaging Institute, Department of Diagnostic, Molecular and Interventional Radiology, and Graduate School of Biomedical Sciences, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
John F CraryDepartment of Neuroscience, Icahn School of Medicine at Mount Sinai, New York, New York, USA.
Allen Institute for Brain Science · USIcahn School of Medicine at Mount Sinai · USColumbia University · US

Funding

Cell type atlasing of whole human brains using HOLiS: an optimized pipeline for staining, clearing, imaging, and analysisRF1MH128969 · NIMH · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI HILLMAN, ELIZABETH M. C., OSTEN, PAVEL · 2021 to 2021
$9.1M
NIMH NIH HHS RF1 MH128969
6 · The paper itself

Abstract

Perfusion fixation is a well-established technique in animal research to improve preservation quality in the study of many tissues, including the brain. There is a growing interest in using perfusion to fix postmortem human brain tissue to achieve the highest fidelity preservation for downstream high-resolution morphomolecular brain mapping studies. Numerous practical barriers arise when applying perfusion fixation in brain banking settings, including the large mass of the organ, degradation of vascular integrity and patency prior to the start of the procedure, and differing investigator goals sometimes necessitating part of the brain to be frozen. As a result, there is a critical need to establish a perfusion fixation procedure in brain banking that is flexible and scalable. This technical report describes our approach to developing an

Indexed as

Brain bankingEx vivo neuroimagingPerfusion fixationPerfusion pressurePostmortem intervalRNAscopeTissue morphology

Identifiers

PMID37284166
PMCPMC10209847
OpenAlexW4379600492

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.