Evidence map›Paper›PMID 37283891›Full record

ArticlePeerJ2023

Influence of SPIO labelling on the function of BMSCs in chemokine receptors expression and chemotaxis.

Yuanchun Liu, Wanyi Huang, Huiyang Wang, Wei Lu, Jiayu Guo, Li Yu, Lina Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PeerJ, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.9field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 3 citations in OpenAlex.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 1 country.

Yuanchun LiuDepartment of Pediatrics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.
Wanyi HuangDepartment of Pediatrics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.
Huiyang WangDepartment of Pediatrics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.
Wei LuDepartment of Pediatrics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.
Jiayu GuoDepartment of Pediatrics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.
Li YuDepartment of Pediatrics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.
Lina WangDepartment of Pediatrics, Guangzhou First People's Hospital, School of Medicine, South China University of Technology, Guangzhou, China.
Guangzhou Medical University · CNGuangzhou First People's Hospital · CNSouth China University of Technology · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bone marrow-derived mesenchymal stem cells (BMSCs) are increasingly being used in bone marrow transplantation (BMT) to enable homing of the allogeneic hematopoietic stem cells and suppress acute graft versus host disease (aGVHD). The aim of this study was to optimize the labelling of BMSCs with superparamagnetic iron oxide particles (SPIOs), and evaluate the impact of the SPIOs on the biological characteristics, gene expression profile and chemotaxis function of the BMSCs. The viability and proliferation rates of the SPIO-labeled BMSCs were analyzed by trypan blue staining and CCK-8 assay respectively, and the chemotaxis function was evaluated by the transwell assay. The expression levels of chemokine receptors were measured by RT-PCR and flow cytometry. The SPIOs had no effect on the viability of the BMSCs regardless of the labelling concentration and culture duration. The labelling rate of the cells was higher when cultured for 48 h with the SPIOs. Furthermore, cells labeled with 25 µg/ml SPIOs for 48 h had the highest proliferation rates, along with increased expression of chemokine receptor genes and proteins. However, there was no significant difference between the chemotaxis function of the labeled and unlabeled BMSCs. To summarize, labelling BMSCs with 25 µg/ml SPIOs for 48h did not affect their biological characteristics and chemotaxis function, which can be of significance for

Indexed as

Magnetite NanoparticlesChemotaxisFerric CompoundsMagnetic Resonance ImagingFerric Compoundsferric oxideMagnetite NanoparticlesaGVHDBMSCChemokine receptorChemotaxisSPIO

Identifiers

PMID37283891
PMCPMC10241165
OpenAlexW4379185265

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.