Evidence map›Paper›PMID 37283805›Full record

ArticleBiomaterials and biosystems2023

Mesenchymal stromal cell exosomes enhance dental pulp cell functions and promote pulp-dentin regeneration.

Jiajun Shi, Kristeen Ye Wen Teo, Shipin Zhang, Ruenn Chai Lai, Vinicius Rosa, Huei Jinn Tong, Mandeep S Duggal, Sai Kiang Lim, Wei Seong Toh

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomaterials and biosystems, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers, 3 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
25citing papers in PubMed, 3 pooled it
3.1field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

25 citing papers in PubMed, 3 syntheses or guidelines pooled it, 20 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Jiajun ShiFaculty of Dentistry, National University of Singapore, Singapore.
Kristeen Ye Wen TeoFaculty of Dentistry, National University of Singapore, Singapore.
Shipin ZhangFaculty of Dentistry, National University of Singapore, Singapore.
Ruenn Chai LaiInstitute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore.
Vinicius RosaFaculty of Dentistry, National University of Singapore, Singapore.
Huei Jinn TongFaculty of Dentistry, National University of Singapore, Singapore.
Mandeep S DuggalCollege of Dental Medicine, QU Health, Qatar University, Qatar.
Sai Kiang LimInstitute of Molecular and Cell Biology, Agency for Science, Technology and Research, Singapore.
Wei Seong TohFaculty of Dentistry, National University of Singapore, Singapore.
National University of Singapore · SGAgency for Science, Technology and Research · SGQatar University · QA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Mesenchymal stromal/stem cell (MSC) therapies are currently being explored for dental pulp regeneration. As the therapeutic effects of MSCs in tissue repair are mediated mainly through the release of extracellular vesicles (EVs) including exosomes, we investigated here the cellular processes and molecular mechanisms modulated by MSC exosomes in dental pulp regeneration. Using dental pulp cell (DPC) cultures, we demonstrated that MSC exosomes could increase DPC migration, proliferation, and odontogenic differentiation. The enhancement of these cellular processes was mediated through exosomal CD73-mediated adenosine receptor activation of AKT and ERK signaling. Consistent with these observations, MSC exosomes increased the expression of dentin matrix proteins and promoted the formation of dentin-like tissue and bridge-like structures in a rat pulp defect model. These effects were comparable to that of mineral trioxide aggregate (MTA) treatment. MSC exosomes also yielded recellularized pulp-dentin tissues in the root canal of endodontically-treated human premolars, following subcutaneous implantation in the mouse dorsum. Together, our findings suggest that MSC exosomes could exert a multi-faceted effect on DPC functions including migration, proliferation and odontogenic differentiation to promote dental pulp regeneration. This study provides the basis for development of MSC exosomes as a cell-free MSC therapeutic alternative for pulp-dentin regeneration.

Indexed as

Dental pulpExosomesExtracellular vesiclesMesenchymal stromal/stem cellsRegeneration

Identifiers

PMID37283805
PMCPMC10239699
OpenAlexW4378471332

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.