Evidence map›Paper›PMID 37281116›Full record

ArticleDevelopment, growth & differentiation1996

Isolation of the Xenopus nrp-1 gene and analysis of its expression following the midblastula transition in injected Xenopus embryos.

Marcia L O'Connell, Martha L Rebbert, Igor B Dawid

Open access · bronzeAbstract read
PubMed Publisher
In one paragraph

Article in Development, growth & differentiation, 1996. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.1field-weighted citation impact, top 54% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Marcia L O'ConnellLaboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Martha L RebbertLaboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Igor B DawidLaboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
Eunice Kennedy Shriver National Institute of Child Health and Human Development · USNational Institutes of Health · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

During embryogenesis the differentiation of distinct tissues is marked by the expression of tissue-specific genes. In Xenopus, the neural-specific gene nrp-1 is activated following the midblastula transition (MBT), and is then expressed exclusively in developing neural tissue. To pursue an investigation of the transcriptional regulation of nrp-1, the genomic DNA encoding nrp-1 was isolated and its structure analyzed. Nrp-1 lacks a canonical TATA box proximal to the start site of transcription, but the 5' flanking sequence is highly GC-rich. Injection studies with nrp-1/chloramphenicol acetyltransferase and luciferase constructs demonstrate that in Xenopus embryos, 200 nt of the 5' flanking sequence of nrp-1 are sufficient to drive the expression of reporter genes soon after the MBT, albeit not in a tissue-specific manner. This expression is enhanced by the inclusion of larger regions of the nrp-1 gene.

Indexed as

midblastula transitionneural geneRNA-binding proteintranscriptionXenopus

Identifiers

PMID37281116
OpenAlexW2039779129

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.