ArticleThe Plant cell2023
PUB25 and PUB26 dynamically modulate ICE1 stability via differential ubiquitination during cold stress in Arabidopsis.
Article in The Plant cell, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 89 citations in OpenAlex.
- A GWAS-identified U-box E3 ligase MsPUB6 is positively regulated by MsDREB1C and contributes to drought tolerance in alfalfa.Plant physiology · 2026Article
- E3 ubiquitin ligases in plant defense: mechanisms and prospects for resistance breeding.Science China. Life sciences · 2026Review
- MYB Factors: Hubs of Plant Stress and Hormone Crosstalk.Plant biotechnology journal · 2026Review
- OTUD6A-Mediated Deubiquitination of PRDX1 Protects Against Oral Ulcer by Restoring Mitochondrial Function.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Salvianolic acid B suppresses non-small cell lung cancer growth by promoting Nrf2 proteasomal degradation and triggering autophagy-dependent ferroptosis.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Research Progress on Histone Modification Regulation Mechanisms and Breeding Applications in Plant Abiotic Stress Responses.Plants (Basel, Switzerland) · 2026Review
- Intermolecular-Interaction-Driven Adaptive Remodeling: A Network Perspective on Plant Abiotic Stress Responses.Plants (Basel, Switzerland) · 2026Review
- Genome-wide identification and cold stress expression of inducer of CBF expression (ICE) transcription factors in Taraxacum kok-saghyz.Functional & integrative genomics · 2026Article
- AhNPR4B Interacts with AhPR2-Like and May Contribute to Disease Resistance and Cold Tolerance in Peanut.Plants (Basel, Switzerland) · 2026Article
- Article
- Identification and characterization of U-box E3 ubiquitin ligase genes (ChPUBs) in Cardamine hupingshanensis under abiotic stress.BMC genomics · 2026Article
- Domestication of Tartary Buckwheat Shaped a Regulatory Module for Seedling Salt Tolerance by Targeting the Magnesium Transporter Gene FtMGT2.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Frost fighters: The discovery of a tripartite molecular module that confers cold stress tolerance in apple.The Plant cell · 2026Article
- Post-translational modifications play indispensable roles in cold stress responses of plants.Frontiers in plant science · 2026Review
- Plant responses to cold stress: molecular and physiological adaptive mechanisms with breeding advances.Frontiers in plant science · 2026Review
- Molecular mechanisms of plant freezing tolerance: from cold signal perception to adaptive responses.Frontiers in plant science · 2026Review
- E3 ubiquitin ligases in bone homeostasis: from regulatory mechanisms to skeletal diseases and therapeutic targeting.Frontiers in cell and developmental biology · 2026Review
- The alfalfa U-box E3 ligase MsPUB210 interacts with MsICE1 and positively regulates cold tolerance in transgenic Arabidopsis.Frontiers in plant science · 2026Article
- Multifaceted roles of SCRM/ICE1 in stomatal development, cold stress and beyond.Frontiers in plant science · 2026Review
- Dissecting usuranme biquitin-mediated circadian clock regulation in Arabidopsis via high-throughput yeast screens.Journal of experimental botany · 2025Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ubiquitination modulates protein turnover or activity depending on the number and location of attached ubiquitin (Ub) moieties. Proteins marked by a lysine 48 (K48)-linked polyubiquitin chain are usually targeted to the 26S proteasome for degradation; however, other polyubiquitin chains, such as those attached to K63, usually regulate other protein properties. Here, we show that 2 PLANT U-BOX E3 ligases, PUB25 and PUB26, facilitate both K48- and K63-linked ubiquitination of the transcriptional regulator INDUCER OF C-REPEAT BINDING FACTOR (CBF) EXPRESSION1 (ICE1) during different periods of cold stress in Arabidopsis (Arabidopsis thaliana), thus dynamically modulating ICE1 stability. Moreover, PUB25 and PUB26 attach both K48- and K63-linked Ub chains to MYB15 in response to cold stress. However, the ubiquitination patterns of ICE1 and MYB15 mediated by PUB25 and PUB26 differ, thus modulating their protein stability and abundance during different stages of cold stress. Furthermore, ICE1 interacts with and inhibits the DNA-binding activity of MYB15, resulting in an upregulation of CBF expression. This study unravels a mechanism by which PUB25 and PUB26 add different polyubiquitin chains to ICE1 and MYB15 to modulate their stability, thereby regulating the timing and degree of cold stress responses in plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.