Evidence map›Paper›PMID 37278916›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2024

Single-Cell mRNA-sncRNA Co-sequencing of Preimplantation Embryos.

Savana Biondic, Cheng Zhao, Michael Hagemann-Jensen, Stewart J Russell, Katherine Vandal, Jesica Canizo, Clifford L Librach, Sophie Petropoulos

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.7field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 2 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 2 countries.

Savana BiondicDépartement de Médecine, Université de Montréal, Montréal, QC, Canada.
Cheng ZhaoDepartment of Clinical Science, Intervention and Technology, Karolinska Institutet, and Division of Obstetrics and Gynecology, Karolinska. Universitetssjukhuset, Stockholm, Sweden.
Michael Hagemann-JensenDepartment of Cell and Molecular Biology, Karolinska Institutet, Stockholm, Sweden.
Stewart J RussellCReATe Fertility Centre, Toronto, ON, Canada.
Katherine VandalDépartement de Médecine, Université de Montréal, Montréal, QC, Canada.
Jesica CanizoDépartement de Médecine, Université de Montréal, Montréal, QC, Canada.
Clifford L LibrachCReATe Fertility Centre, Toronto, ON, Canada.
Sophie PetropoulosDépartement de Médecine, Université de Montréal, Montréal, QC, Canada.
Centre Hospitalier de l’Université de Montréal · CAKarolinska Institutet · SECReATe Fertility Centre · CAKarolinska University Hospital · SESunnybrook Hospital · CA

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The development of single-cell multiomics has provided the ability to systematically investigate cellular diversity and heterogeneity in different biological systems via comprehensive delineations of individual cellular states. Single-cell RNA sequencing in particular has served as a powerful tool to the study of the molecular circuitries underlying preimplantation embryonic development in both the mouse and human. Here we describe a method to elucidate the cellular dynamics of the embryo further by performing both single-cell RNA sequencing (Smart-Seq2) and single-cell small non-coding RNA sequencing (Small-Seq) on the same individual embryonic cell.

Indexed as

RNA, Small UntranslatedAnimalsBlastocystEmbryo, MammalianEmbryonic DevelopmentFemaleHumansMicePregnancyRNA, MessengerRNA, MessengerRNA, Small UntranslatedBlastocystsCo-SeqEmbryosPreimplantation developmentSingle-cell multiomicsSingle-cell RNA sequencingSmall non-coding RNAsSmall-SeqSmart-Seq2

Identifiers

PMID37278916
OpenAlexW4379509235

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.