Evidence map›Paper›PMID 37278603›Full record

ArticleACS synthetic biology2023

Cell-Free Expression System Derived from a Near-Minimal Synthetic Bacterium.

Andrei Sakai, Aafke J Jonker, Frank H T Nelissen, Evan M Kalb, Bob van Sluijs, Hans A Heus, Katarzyna P Adamala, John I Glass, Wilhelm T S Huck

Abstract read
In one paragraph

Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Reconstituting alternative life using the test-bed of cell-free systems.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025
    Review
  5. Building a Synthetic Cell Together.Nature communications · 2025
    Review
  6. Article
  7. Review
  8. Article
  9. Synthetic biology (Oxford, England) · 2024
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Andrei SakaiInstitute for Molecules and Materials, Radboud University, Nijmegen 6525AJ, The Netherlands.ORCID 0000-0002-2700-8059
Aafke J JonkerInstitute for Molecules and Materials, Radboud University, Nijmegen 6525AJ, The Netherlands.
Frank H T NelissenInstitute for Molecules and Materials, Radboud University, Nijmegen 6525AJ, The Netherlands.
Evan M KalbDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, United States.
Bob van SluijsInstitute for Molecules and Materials, Radboud University, Nijmegen 6525AJ, The Netherlands.
Hans A HeusInstitute for Molecules and Materials, Radboud University, Nijmegen 6525AJ, The Netherlands.
Katarzyna P AdamalaDepartment of Genetics, Cell Biology and Development, University of Minnesota, Minneapolis, Minnesota 55455, United States.ORCID 0000-0003-1066-7207
John I GlassSynthetic Biology & Bioenergy, J. Craig Venter Institute, La Jolla, California 92037, United States.
Wilhelm T S HuckInstitute for Molecules and Materials, Radboud University, Nijmegen 6525AJ, The Netherlands.ORCID 0000-0003-4222-5411

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cell-free expression (CFE) systems are fundamental to reconstituting metabolic pathways

Indexed as

BacteriaCell-Free Systemactive machine learningcell-free expression systemJCVI-syn3AMycoplasma

Identifiers

PMID37278603
PMCPMC10278164

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.