Evidence map›Paper›PMID 37277633›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Genetic Code Expansion in Mammalian Cells Through Quadruplet Codon Decoding.

Yan Chen, Tianyu Gao, Xinyuan He, Wei Niu, Jiantao Guo

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.8field-weighted citation impact, top 27% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 1 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Yan ChenDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Tianyu GaoDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA.
Xinyuan HeDepartment of Chemical & Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA.
Wei NiuDepartment of Chemical & Biomolecular Engineering, University of Nebraska-Lincoln, Lincoln, NE, USA. jguo4@unl.edu.
Jiantao GuoDepartment of Chemistry, University of Nebraska-Lincoln, Lincoln, NE, USA. wniu2@unl.edu.
University of Nebraska–Lincoln · US

Funding

Targeted mass spectrometry approaches to understand CART processing and recepter interactionsP20GM113126 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI RAZAVI RIZI, MOHAMMAD · 2016 to 2025
$20.8M
Development of proximity-induced fluorogenic reactions for imaging biomolecular interaction through noncanonical amino acid mutagenesis in response to quadruplet codon and recoding signalR01GM138623 · NIGMS · UNIVERSITY OF NEBRASKA LINCOLN · PI GUO, JIANTAO · 2020 to 2021
$613k
NIGMS NIH HHS R01 GM138623
6 · The paper itself

Abstract

Genetic code expansion enables the site-specific incorporation of noncanonical amino acids (ncAAs) into proteins both in vitro and in vivo. In addition to a widely applied nonsense suppression strategy, the use of quadruplet codons could further expand the genetic code. A general approach to genetically incorporate ncAAs in response to quadruplet codons is achieved by utilizing an engineered aminoacyl-tRNA synthetase (aaRS) together with a tRNA variant containing an expanded anticodon loop. Here we provide a protocol to decode quadruplet UAGA codon with a ncAA in mammalian cells. We also describe microscopy imaging and flow cytometry analysis of ncAA mutagenesis in response to quadruplet codons.

Indexed as

Amino Acyl-tRNA SynthetasesGenetic CodeAmino AcidsAnimalsAnticodonCodonCodon, TerminatorMammalsRNA, TransferAmino AcidsAmino Acyl-tRNA SynthetasesAnticodonCodonCodon, TerminatorRNA, TransferFour-base codonGenetic code expansionNon-canonical amino acidQuadruplet codonUnnatural amino acid

Identifiers

PMID37277633
OpenAlexW4379472248

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.