Evidence map›Paper›PMID 37277627›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Reprogramming Initiator and Nonsense Codons to Simultaneously Install Three Distinct Noncanonical Amino Acids into Proteins in E. coli.

Han-Kai Jiang, Jeffery M Tharp

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Han-Kai JiangDepartment of Molecular Biophysics & Biochemistry, Yale University, New Haven, CT, USA.
Jeffery M TharpDepartment of Biochemistry & Molecular Biology, Indiana University School of Medicine, Indianapolis, IN, USA. jemtharp@iu.edu.

Funding

Genetically encoded bicyclic peptide libraries for the discoveryof novel antiviral agentsR00GM141320 · NIGMS · INDIANA UNIVERSITY INDIANAPOLIS · PI THARP, JEFFERY MICHEAL · 2023 to 2025
$866k
Genetically encoded bicyclic peptide libraries for the discoveryof novel antiviral agentsK99GM141320 · NIGMS · YALE UNIVERSITY · PI THARP, JEFFERY MICHEAL · 2021 to 2021
$95k
NIGMS NIH HHS K99 GM141320NIGMS NIH HHS R00 GM141320
6 · The paper itself

Abstract

Multiple noncanonical amino acids can be installed into proteins in E. coli using mutually orthogonal aminoacyl-tRNA synthetase and tRNA pairs. Here we describe a protocol for simultaneously installing three distinct noncanonical amino acids into proteins for site-specific bioconjugation at three sites. This method relies on an engineered, UAU-suppressing, initiator tRNA, which is aminoacylated with a noncanonical amino acid by Methanocaldococcus jannaschii tyrosyl-tRNA synthetase. Using this initiator tRNA/aminoacyl-tRNA synthetase pair, together with the pyrrolysyl-tRNA synthetase/tRNA

Indexed as

Amino AcidsAmino Acyl-tRNA SynthetasesCodon, NonsenseEscherichia coliProteinsRNA, TransferRNA, Transfer, MetAmino AcidsAmino Acyl-tRNA SynthetasesCodon, NonsenseProteinsRNA, TransferRNA, Transfer, MetBioorthogonal chemistryGenetic code expansionNon-canonical amino acidsPyrrolysyl-tRNA synthetaseSynthetic biology

Identifiers

PMID37277627

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.