ArticleFrontiers in microbiology2023
Multiplex PCR-based next generation sequencing as a novel, targeted and accurate molecular approach for periprosthetic joint infection diagnosis.
Article in Frontiers in microbiology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 61 citations in OpenAlex.
- Comparative diagnostic accuracy of next-generation sequencing in different specimen types for periprosthetic joint infection: A systematic review and meta-analysis.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2026Pooled it
- Application of targeted next-generation sequencing in the etiological diagnosis of peritoneal dialysis-associated peritonitis: a single-center prospective cohort study.International urology and nephrology · 2026Article
- Article
- Improved RNA-based metagenomic sequencing for rapid pathogen detection in pets.BMC veterinary research · 2026Article
- Disseminated Culture-Negative Periprosthetic Knee Infection With Multifocal Septic Arthritis Associated WithCase reports in orthopedics · 2026Article
- Epstein-Barr virus-associated inflammatory polyp of the proximal cervical trachea causing central airway obstruction: a case report.Frontiers in medicine · 2026Article
- Current Concepts in Periprosthetic Joint Infection: Modern Biomarkers, Microbiome Insights, Molecular Diagnostics, and Emerging Prevention Strategies.Orthopedic research and reviews · 2026Review
- Precision diagnosis of preoperative infection in urolithiasis: integrating targeted next-generation sequencing for enhanced accuracy-a multicenter cohort study.BMC infectious diseases · 2025Article
- Toxoplasma gondii infection diagnosed by tNGS in a patient with acute lymphoblastic leukemia: a case report.BMC infectious diseases · 2025Article
- The Ongoing Struggle to Find a Gold Standard for PJI Diagnosis.Reports (MDPI) · 2025Review
- Application of targeted metagenomic next-generation sequencing in pneumonia patients.Microbiology spectrum · 2025Article
- Targeted next-generation sequencing - a promising approach in the diagnosis of Mycobacterium tuberculosis and drug resistance.Infection · 2025Article
- Septic arthritis - symptoms, diagnosis and new therapy.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2025Review
- Diagnosis and surgical treatment of chronic destructive septic hip arthritis.Arthroplasty (London, England) · 2025Review
- High-Throughput Sequencing of Nontuberculous Mycobacterial Flora andVeterinary sciences · 2025Article
- Clinical diagnosis of Q fever by targeted next-generation sequencing for identification of Coxiella burnetii.BMC infectious diseases · 2025Article
- Comparative and clinical impact of targeted next-generation sequencing in pediatric pneumonia diagnosis and treatment.Frontiers in microbiology · 2025Article
- Comparative performance evaluation of FilmArray RP 2.1 and targeted next-generation sequencing in upper respiratory tract infections.Frontiers in cellular and infection microbiology · 2025Article
- A case report of rapid diagnosis ofFrontiers in medicine · 2025Article
- Evaluation of targeted next-generation sequencing for microbiological diagnosis of acute lower respiratory infection.Frontiers in microbiology · 2025Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objectives: Periprosthetic joint infection (PJI) diagnosis remains challenging, and the identification of the causative microorganism is, by far, the most important aspect. Here, we use multiple PCR-based targeted next-generation sequencing (tNGS) to detect pathogens in PJI. To explore 1. the ability of targeted next-generation sequencing (tNGS) to detect pathogens in PJI; 2. the consistency of tNGS, metagenomic NGS (mNGS), and culture results; and 3. the ability of tNGS to detect drug resistance genes in PJI. Methods: PJI was diagnosed according to the Musculoskeletal Infection Society (MSIS) criteria. The microorganisms were detected by culture, mNGS and tNGS to compare the diagnostic effectiveness of the three methods for PJI and to compare their consistency in detecting microorganisms. Drug resistance genes were detected using tNGS. The costs and turnaround times of mNGS and tNGS were compared. Results: Forty-three patients with PJI, 21 patients without PJI and 10 negative control cases were included. The culture, tNGS, and mNGS sensitivities for PJI diagnosis were 74.41%, 88.37%, and 93.02%, respectively, with no significant differences. The specificities were 90.48%, 95.24%, and 95.24%, respectively, with no significant differences. tNGS detected drug resistance genes in 37.5% of culture-positive PJIs. tNGS was superior to mNGS for turnaround time (14.5 h vs. 28 h) and cost ($150 vs. $260). Conclusions: tNGS can effectively identify PJI pathogens and may provide drug resistance information, while tNGS is superior to mNGS regarding cost and turnaround time. A multidisciplinary, multi-technology based algorithm to diagnose PJI is appropriate. Highlights: 298 microorganisms and 86 drug resistance genes were included in the tNGS panel.Diagnostic efficacy of tNGS is not inferior to that of commonly used indicators.tNGS is superior to mNGS in cost and turnaround time.
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