Evidence map›Paper›PMID 37273064›Full record

ArticleMolecular biology reports2023

Molecular characterization of wild-type and HSAN2B-linked FAM134B.

Akane Kanamori, Shohei Hinaga, Yoko Hirata, Fumimasa Amaya, Kentaro Oh-Hashi

Abstract read
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In one paragraph

Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

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5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Akane KanamoriGraduate School of Natural Science and Technology, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
Shohei HinagaGraduate School of Natural Science and Technology, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
Yoko HirataGraduate School of Natural Science and Technology, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan.
Fumimasa AmayaDepartment of Pain Management and Palliative Care Medicine, Kyoto Prefectural University of Medicine, Kawaramachi-Hirokoji, Kamikyo-Ku, Kyoto, 602-0841, Japan.
Kentaro Oh-HashiGraduate School of Natural Science and Technology, Gifu University, 1-1 Yanagido, Gifu, 501-1193, Japan. oh-hashi.kentaro.u7@f.gifu-u.ac.jp.
Gifu University · JPKyoto Prefectural University of Medicine · JP

Funding

Grants-in-aid from the Japan Society for the Promotion of Science 19H04030Grants-in-aid from the Japan Society for the Promotion of Science 20K21751
6 · The paper itself

Abstract

backgroundFamily with sequence similarity 134, member B (FAM134B), also known as Reticulophagy regulator 1 (RETREG1), is an ER-phagy receptor involved in ER homeostasis. Congenital mutations in the FAM134B gene have been reported to be associated with hereditary sensory and autonomic neuropathy type 2B (HSAN2B); however, the molecular differences between wild-type and HSAN2B-linked FAM134B are not fully understood. METHODS AND

resultsWe prepared several human FAM134B constructs, such as the HSAN2B-linked mutant, and compared their features with those of wild-type FAM134B by transfecting these constructs into FAM134B-deficient Neuro2a cells. Although intrinsic FAM134B protein expression in wild-type Neuro2a cells was affected by the supply of amino acids in the culture medium, the expression of each HSAN2B-linked mutant FAM134B protein was hardly affected by serum and amino acid deprivation. On the other hand, the intracellular localization of GFP-tagged HSAN2B-linked mutants, except for P7Gfs133X, overlapped well with ER-localized SP-RFP

conclusionsWe showed that wild-type and HSAN2B-linked FAM134B have different molecular characteristics by transfecting cells with various types of constructs. Thus, this study provides new insights into the molecular mechanisms underlying HSAN2B as well as the regulation of ER-phagy.

Indexed as

Hereditary Sensory and Autonomic NeuropathiesIntracellular Signaling Peptides and ProteinsAutophagyHumansMembrane ProteinsIntracellular Signaling Peptides and ProteinsMembrane ProteinsRETREG1 protein, humanER-phagyFAM134BHSAN2B

Identifiers

PMID37273064
OpenAlexW4379376596

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.