ArticleDevelopment (Cambridge, England)2023
Tools to analyze the organization and formation of the germline cyst in zebrafish oogenesis.
Article in Development (Cambridge, England), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 11 citations in OpenAlex.
- Protocol for culture of zebrafishdeveloping ovaries as an ex vivo system for investigating early oogenesis and ovarian development.STAR protocols · 2026Article
- Evaluating the Effects of Neburon Exposure on Ovarian Folliculogenesis Using Zebrafish and Mouse Granulosa Cell Line.Environmental health perspectives · 2026Article
- An mTOR-Stat3-Stathmin pathway controls centrosome and microtubule dynamics for oocyte polarization.Current biology : CB · 2025Article
- Early female germline development inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Three types of actomyosin rings within a common cytoplasm exhibit distinct modes of contractility.Molecular biology of the cell · 2025Article
- Three types of actomyosin rings within a common cytoplasm exhibit distinct modes of contractility.bioRxiv : the preprint server for biology · 2025Article
- A tug-of-war between germ cell motility and intercellular bridges controls germline cyst formation in mice.Current biology : CB · 2024Article
- Zebrafish as a model system for studying reproductive diseases.Frontiers in cell and developmental biology · 2024Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Oocytes develop in the germline cyst, a cellular organization in which germ cells are tightly interconnected and surrounded by somatic cells. The cyst produces oocytes for follicle formation and is a hub for essential processes in meiosis and oocyte differentiation. However, the formation and organization of the cyst, and their contribution to oocyte production in vertebrates remain unclear. Here, we provide tools for three-dimensional and functional in vivo analyses of the germline cyst in the zebrafish ovary. We describe the use of serial block-face scanning electron microscopy (SBF-SEM) to resolve the three-dimensional architecture of cells and organelles in the cyst at ultrastructural resolution. We present a deep learning-based pipeline for high-throughput quantitative analysis of three-dimensional confocal datasets of cysts in vivo. We provide a method for laser ablation of cellular components for manipulating cyst cells in ovaries. These methods will facilitate the investigation of the cyst cellular organization, expand the toolkit for the study of the zebrafish ovary, and advance our understanding of female developmental reproduction. They could also be further applied to the investigation of other developmental systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.