ArticleThe Journal of allergy and clinical immunology2023
Asthma-protective agents in dust from traditional farm environments.
Article in The Journal of allergy and clinical immunology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The trial behind it
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Who cites it
11 citing papers in PubMed, 19 citations in OpenAlex.
- What health are we talking about? Biodiversity as the missing link between One Health and Planetary Health.Ambio · 2026Article
- Early Life Exposure to Air Pollutants and Childhood Asthma.Current allergy and asthma reports · 2026Review
- Update on environmental determinants of allergic diseases.The Journal of allergy and clinical immunology · 2026Review
- The Role of the Indoor Exposome in Food Allergy Development.Current allergy and asthma reports · 2026Review
- The Therapeutic Potential of Farm Dust Extracts in a Mouse Model of Eosinophilic Inflammation.Allergy · 2026Article
- A beneficial environment promotes immune resilience through epigenetic regulation.Science advances · 2026Article
- Risk Factors and Mechanisms Leading to Preschool Recurrent Wheeze and Asthma.The journal of allergy and clinical immunology. In practice · 2025Review
- Harnessing the Farm Effect: Microbial Products for the Treatment and Prevention of Asthma Throughout Life.Immunological reviews · 2025Review
- Microbial influencers: the airway microbiome's role in asthma.The Journal of clinical investigation · 2025Review
- Gradient disparities in allergy and the gut microbiome among rural, migrant, and urban populations across China.The World Allergy Organization journal · 2025Article
- Administration of a bacterial lysate to the airway compartment is sufficient to inhibit allergen-induced lung eosinophilia in germ-free mice.Journal of leukocyte biology · 2024Article
Corrections and comments
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Authors and funding
23 authors at 5 institutions in 3 countries.
Funding
Abstract
backgroundGrowing up on traditional European or US Amish dairy farms in close contact with cows and hay protects children against asthma, and airway administration of extracts from dust collected from cowsheds of those farms prevents allergic asthma in mice.
objectivesThis study sought to begin identifying farm-derived asthma-protective agents.
methodsOur work unfolded along 2 unbiased and independent but complementary discovery paths. Dust extracts (DEs) from protective and nonprotective farms (European and Amish cowsheds vs European sheep sheds) were analyzed by comparative nuclear magnetic resonance profiling and differential proteomics. Bioactivity-guided size fractionation focused on protective Amish cowshed DEs. Multiple in vitro and in vivo functional assays were used in both paths. Some of the proteins thus identified were characterized by in-solution and in-gel sodium dodecyl sulfate-polyacrylamide gel electrophoresis enzymatic digestion/peptide mapping followed by liquid chromatography/mass spectrometry. The cargo carried by these proteins was analyzed by untargeted liquid chromatography-high-resolution mass spectrometry.
resultsTwelve carrier proteins of animal and plant origin, including the bovine lipocalins Bos d 2 and odorant binding protein, were enriched in DEs from protective European cowsheds. A potent asthma-protective fraction of Amish cowshed DEs (≈0.5% of the total carbon content of unfractionated extracts) contained 7 animal and plant proteins, including Bos d 2 and odorant binding protein loaded with fatty acid metabolites from plants, bacteria, and fungi.
conclusionsAnimals and plants from traditional farms produce proteins that transport hydrophobic microbial and plant metabolites. When delivered to mucosal surfaces, these agents might regulate airway responses.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.