Evidence map›Paper›PMID 37268984›Full record

ArticleMalaria journal2023

Detection of Plasmodium simium gametocytes in non-human primates from the Brazilian Atlantic Forest.

Lara Cotta Amaral, Yanka Evellyn Alves Rodrigues Salazar, Denise Anete Madureira de Alvarenga, Anielle de Pina-Costa, Ana Júlia Dutra Nunes, Júlio Cesar de Souza Junior, Gustavo Henrique Pereira Gonçalves, Zelinda Maria Braga Hirano, Sílvia Bahadian Moreira, Alcides Pissinatti and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Malaria journal, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.4field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Lara Cotta AmaralGrupo de Pesquisa em Biologia Molecular e Imunologia da Malária, Instituto René Rachou, Fundação Oswaldo Cruz (Fiocruz), Belo Horizonte, Brazil.
Yanka Evellyn Alves Rodrigues SalazarGrupo de Pesquisa em Biologia Molecular e Imunologia da Malária, Instituto René Rachou, Fundação Oswaldo Cruz (Fiocruz), Belo Horizonte, Brazil.
Denise Anete Madureira de AlvarengaGrupo de Pesquisa em Biologia Molecular e Imunologia da Malária, Instituto René Rachou, Fundação Oswaldo Cruz (Fiocruz), Belo Horizonte, Brazil.
Anielle de Pina-CostaLaboratório de Doenças Febris Agudas, Instituto Nacional de Infectologia Evandro Chagas, Fiocruz, Rio de Janeiro, Brazil.
Ana Júlia Dutra NunesPrograma de Conservação do Bugio Ruivo, Joinville, Brazil.
Júlio Cesar de Souza JuniorCentro de Pesquisas Biológicas de Indaial, Indaial, Brazil.
Gustavo Henrique Pereira GonçalvesCentro de Pesquisas Biológicas de Indaial, Indaial, Brazil.
Zelinda Maria Braga HiranoCentro de Pesquisas Biológicas de Indaial, Indaial, Brazil.
Sílvia Bahadian MoreiraCentro de Primatologia do Rio de Janeiro/INEA, Guapimirim, Brazil.
Alcides PissinattiCentro de Primatologia do Rio de Janeiro/INEA, Guapimirim, Brazil.
Cláudio Tadeu Daniel-RibeiroCentro de Pesquisa, Diagnóstico e Treinamento em Malária, Fiocruz, Rio de Janeiro, Brazil.
Taís Nóbrega de SousaGrupo de Pesquisa em Biologia Molecular e Imunologia da Malária, Instituto René Rachou, Fundação Oswaldo Cruz (Fiocruz), Belo Horizonte, Brazil.
Cristiana Ferreira Alves de BritoGrupo de Pesquisa em Biologia Molecular e Imunologia da Malária, Instituto René Rachou, Fundação Oswaldo Cruz (Fiocruz), Belo Horizonte, Brazil. cristiana.brito@fiocruz.br.
Fundação Oswaldo Cruz · BRUniversidade Regional de Blumenau · BRCentro Universitário Serra dos Órgãos · BRUniversidade Federal Fluminense · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundPlasmodium species of non-human primates (NHP) are of great interest because they can naturally infect humans. Plasmodium simium, a parasite restricted to the Brazilian Atlantic Forest, was recently shown to cause a zoonotic outbreak in the state of Rio de Janeiro. The potential of NHP to act as reservoirs of Plasmodium infection presents a challenge for malaria elimination, as NHP will contribute to the persistence of the parasite. The aim of the current study was to identify and quantify gametocytes in NHP naturally-infected by P. simium.

methodsWhole blood samples from 35 NHP were used in quantitative reverse transcription PCR (RT-qPCR) assays targeting 18S rRNA, Pss25 and Pss48/45 malaria parasite transcripts. Absolute quantification was performed in positive samples for 18S rRNA and Pss25 targets. Linear regression was used to compare the quantification cycle (Cq) and the Spearman's rank correlation coefficient was used to assess the correlation between the copy numbers of 18S rRNA and Pss25 transcripts. The number of gametocytes/µL was calculated by applying a conversion factor of 4.17 Pss25 transcript copies per gametocyte.

resultsOverall, 87.5% of the 26 samples, previously diagnosed as P. simium, were positive for 18S rRNA transcript amplification, of which 13 samples (62%) were positive for Pss25 transcript amplification and 7 samples (54%) were also positive for Pss48/45 transcript. A strong positive correlation was identified between the Cq of the 18S rRNA and Pss25 and between the Pss25 and Pss48/45 transcripts. The 18S rRNA and Pss25 transcripts had an average of 1665.88 and 3.07 copies/µL, respectively. A positive correlation was observed between the copy number of Pss25 and 18S rRNA transcripts. Almost all gametocyte carriers exhibited low numbers of gametocytes (< 1/µL), with only one howler monkey having 5.8 gametocytes/µL.

conclusionsFor the first time, a molecular detection of P. simium gametocytes in the blood of naturally-infected brown howler monkeys (Alouatta guariba clamitans) was reported here, providing evidence that they are likely to be infectious and transmit P. simium infection, and, therefore, may act as a reservoir of malaria infection for humans in the Brazilian Atlantic Forest.

Indexed as

MalariaPlasmodiumAlouattaAnimalsBrazilForestsHumansPlasmodium falciparumPrimatesRNA, Ribosomal, 18SRNA, Ribosomal, 18SGametocytesInfectious disease transmissionMalariaNon-human primatePlasmodium simiumZoonoses

Identifiers

PMID37268984
PMCPMC10239093
OpenAlexW4379197687

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.