ArticlePLoS pathogens2023
Targeted IS-element sequencing uncovers transposition dynamics during selective pressure in enterococci.
Article in PLoS pathogens, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Insertion sequence elements associated with Staphylococcus epidermidis evolution in persistent orthopaedic device-related infections.BMC genomics · 2026Article
- Genetic determinants of disinfectant and antibiotic resistance, and virulence factors in Enterococcus faecalis isolated from a pasteurized crab canning environment.Antonie van Leeuwenhoek · 2026Article
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- Functional characterization of macrolide esterase from cyanobacteria and their potential dissemination risk.npj antimicrobials and resistance · 2026Article
- Linezolid and levofloxacin: an uncommon pairing that suppresses evolution of resistance inFEMS microbes · 2026Article
- Independent, ongoing clade-specific expansions of ISMicrobial genomics · 2026Article
- zol and fai: large-scale targeted detection and evolutionary investigation of gene clusters.Nucleic acids research · 2025Article
- zol & fai: large-scale targeted detection and evolutionary investigation of gene clusters.bioRxiv : the preprint server for biology · 2024Article
- Local Genomic Surveillance of InvasiveInternational journal of molecular sciences · 2024Article
- A metagenomics pipeline reveals insertion sequence-driven evolution of the microbiota.Cell host & microbe · 2024Article
- The virtue of training: extending phage host spectra against vancomycin-resistantAntimicrobial agents and chemotherapy · 2024Article
- A metagenomics pipeline reveals insertion sequence-driven evolution of the microbiota.bioRxiv : the preprint server for biology · 2024Article
- Current Knowledge of Enterococcal Endocarditis: A Disease Lurking in Plain Sight of Health Providers.Pathogens (Basel, Switzerland) · 2024Review
- Emerging Issues and Initial Insights into Bacterial Biofilms: From Orthopedic Infection to Metabolomics.Antibiotics (Basel, Switzerland) · 2024Review
- Genome reduction inFrontiers in bioengineering and biotechnology · 2024Article
- Comparative genomics reveals the correlations of stress response genes and bacteriophages in developing antibiotic resistance ofmSystems · 2023Article
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Authors and funding
8 authors at 3 institutions in 1 country.
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Abstract
Insertion sequences (IS) are simple transposons implicated in the genome evolution of diverse pathogenic bacterial species. Enterococci have emerged as important human intestinal pathogens with newly adapted virulence potential and antibiotic resistance. These genetic features arose in tandem with large-scale genome evolution mediated by mobile elements. Pathoadaptation in enterococci is thought to be mediated in part by the IS element IS256 through gene inactivation and recombination events. However, the regulation of IS256 and the mechanisms controlling its activation are not well understood. Here, we adapt an IS256-specfic deep sequencing method to describe how chronic lytic phage infection drives widespread diversification of IS256 in E. faecalis and how antibiotic exposure is associated with IS256 diversification in E. faecium during a clinical human infection. We show through comparative genomics that IS256 is primarily found in hospital-adapted enterococcal isolates. Analyses of IS256 transposase gene levels reveal that IS256 mobility is regulated at the transcriptional level by multiple mechanisms in E. faecalis, indicating tight control of IS256 activation in the absence of selective pressure. Our findings reveal that stressors such as phages and antibiotic exposure drives rapid genome-scale transposition in the enterococci. IS256 diversification can therefore explain how selective pressures mediate evolution of the enterococcal genome, ultimately leading to the emergence of dominant nosocomial lineages that threaten human health.
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