Evidence map›Paper›PMID 37266879›Full record

ArticleBiotechnology letters2023

Construction of a food-grade gene editing system based on CRISPR-Cas9 and its application in Lactococcus lactis NZ9000.

Yangping Zhou, Fei Song, Hongru Yang, Dongyao Li, Na Zhang, Kunlun Huang, Xiaoyun He, Miaoshu Wang, Hongtao Tian, Chen Li

Abstract read
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In one paragraph

Article in Biotechnology letters, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Pharmaceutics · 2026
    Review
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  4. Article
  5. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Yangping Zhou *College of Food Science and Technology, Hebei Agricultural University, Baoding, 071000, Hebei, China.
Fei Song *College of Food Science and Technology, Hebei Agricultural University, Baoding, 071000, Hebei, China.
Hongru Yang *College of Food Science and Technology, Hebei Agricultural University, Baoding, 071000, Hebei, China.
Dongyao LiCollege of Food Science and Technology, Hebei Agricultural University, Baoding, 071000, Hebei, China.
Na ZhangCollege of Food Science and Technology, Hebei Agricultural University, Baoding, 071000, Hebei, China.
Kunlun HuangKey Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture, Beijing, 100083, China.
Xiaoyun HeKey Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety), Ministry of Agriculture, Beijing, 100083, China.
Miaoshu WangHebei Technology Innovation Center of Probiotic Functional Dairy Product, Baoding, 071000, Hebei, China.
Hongtao TianCollege of Food Science and Technology, Hebei Agricultural University, Baoding, 071000, Hebei, China. tht631022@163.com.ORCID http://orcid.org/0000-0002-3079-0125
Chen LiCollege of Food Science and Technology, Hebei Agricultural University, Baoding, 071000, Hebei, China. yitai123@163.com.

Funding

Opening Project of Key Laboratory of Safety Assessment of Genetically Modified Organism (Food Safety) from Ministry of Agriculture and Rural Affairs of the People's Republic of China SAGM-Y-2017006the Food Processing Discipline Group of Hebei Agricultural University Grant No. 2021-08the Key Research and Development Program of Hebei Province Grant No. 19227134Dthe Science and Technology Research Projects of Colleges and Universities in Hebei province Grant No. ZD2021059
6 · The paper itself

Abstract

Clustered regularly interspaced short palindromic repeats-associated protein 9 (CRISPR/Cas9) system has been widely used in gene editing of various organisms. However, food-grade gene editing systems in lactic acid bacteria are still preliminary. Red/ET-dependent homologous recombination or CRISPR-based systems have been developed to gene editing in Lactococcus lactis, but these methods are overall inefficient. In the present study, a recombinant system based on CRISPR/Cas9 technology combined with Red/ET was developed using the plasmid pMG36e derived from Lactococcus lactis. Then, the developed recombinant system was applied to Lactococcus lactis. Knockout efficiency was significantly higher using the developed system (91%). In addition, this system showed the potential to be used as a high-throughput method for hierarchical screening. Finally, a gene-edited strain was obtained, and no antibiotics or exogenous genes were introduced using the developed gene editing system. Thus, the efficient system in lactic acid bacteria was constructed and optimized.

Indexed as

Gene EditingLactococcus lactisCRISPR-Cas SystemsHomologous RecombinationPlasmidsCRISPRGene editingGenomic engineeringLactate dehydrogenaseLactococcus lactis

Identifiers

PMID37266879

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.