ArticleBMC plant biology2023
Optimal protocol for in vitro polyploid induction of Cymbidium aloifolium (L.) Sw.
Article in BMC plant biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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Who cites it
4 citing papers in PubMed.
- In vitro induction of tetraploids and their morphological and phenolic acid content in Cnidium officinale.BMC plant biology · 2026Article
- Insight of the seed germination of Lily species under different chemical treatments and light conditions.Scientific reports · 2025Article
- Induction of Autopolyploidy and Preliminary Investigation of the Dwarfing Mechanism inPlants (Basel, Switzerland) · 2025Article
- The Role of Colchicine in Plant Breeding.International journal of molecular sciences · 2025Review
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Authors and funding
5 authors.
Funding
Abstract
backgroundCymbidium aloifolium is a popular ornamental flower in Thailand with both economic and medical values. Polyploid induction techniques are used to improve plant quality. This study identified polyploidy levels of C. aloifolium induction by colchicine. Protocorms of C. aloifolium were treated on solid New Dogashima Medium (NDM) with various concentrations of colchicine (0, 0.01, 0.02, 0.03, 0.04 and 0.05% w/v) for 2, 4 and 8 weeks.
resultsColchicine effectively induced plant polyploidy. Tetraploid plants were found after treatment with 0.03% and 0.04% colchicine for 8 weeks, while at increased concentration and duration, survival, response and growth performance decreased. Tetraploid plants showed the lowest growth performance but highest size of stomatal and epidermal cells. Growth performance and leaf surface anatomy data were analyzed by Pearson's correlation and PCA. Results showed that stomatal and epidermal cell sizes had strongly negative correlations with other variables, while HCA revealed that stomatal and epidermal cell sizes of tetraploid plantlets were larger but stomatal and epidermal cell densities decreased when compared with the diploids.
conclusionColchicine at suitable concentrations and duration produced polyploid plants with alteration of morphological and anatomical traits. This study provides potential information to support orchid quality production as ornamental plants and a source of pharmaceutical raw materials.
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