ArticleReproductive sciences (Thousand Oaks, Calif.)2023
Targeted Amino Acids Profiling of Human Seminal Plasma from Teratozoospermia Patients Using LC-MS/MS.
Article in Reproductive sciences (Thousand Oaks, Calif.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Novel HPLC-laser-stimulated fluorescence profiling of seminal plasma amino acids linked to ejaculate quality.Journal of assisted reproduction and genetics · 2026Article
- Article
- Effectiveness of Curcumin on Oxidative Stress in Goat Semen: Explorations Regarding Semen Quality, Sperm Apoptosis, Ultrastructure, and Markers of Oxidative Stress.Antioxidants (Basel, Switzerland) · 2025Article
- Integrated seminal plasma metabolomics and lipidomics profiling highlight distinctive signature of varicocele patients with male infertility.Asian journal of andrology · 2025Article
- Evaluation of Ambient Ozone Pollution Exposure on Mice Testicular Tissue: An Integrated Metabolomic and Transcriptomic Analysis.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Branched-chain and Aromatic Amino Acids in Blood and Seminal Plasma are Associated with Sperm Parameters; A Practice within a Fertility Clinic Considering the Metabolic Syndrome.Reproductive sciences (Thousand Oaks, Calif.) · 2025Article
- Untargeted metabolomics fingerprints in seminal plasma of patients with abnormal sperm morphology using high-performance liquid chromatography and mass spectrometry.Frontiers in molecular biosciences · 2025Article
- Metabolomic profiling of human semen in patients with oligospermia using high performance liquid chromatography-tandem mass spectrometry.Scientific reports · 2024Article
- Critical Factors in Sample Collection and Preparation for Clinical Metabolomics of Underexplored Biological Specimens.Metabolites · 2024Review
- Article
- The Amino Acid Profile in Seminal Plasma of Normozoospermic Men: A Correlation Analysis with Spermiogram Parameters and Total Antioxidant Capacity.Journal of reproduction & infertilityArticle
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
Identifying the metabolome of human seminal plasma (HSP) is a new research area to screen putative biomarkers of infertility. This case-control study was performed on HSP specimens of 15 infertile patients with teratozoospermia (defined as normal sperm morphology < 4%) and 12 confirmed fertile normozoospermic men as the control group to investigate the seminal metabolic signature and whether there are differences in the metabolome between two groups. HSPs were subjected to LC-MS-MS analysis. MetaboAnalyst5.0 software was utilized for statistical analysis. Different univariate and multivariate analyses were used, including T-tests, fold change analysis, random forest (RF), and metabolite set enrichment analysis (MSEA). Teratozoospermic samples contained seventeen significantly different amino acids. Upregulated metabolites include glutamine, asparagine, and glycylproline, whereas downregulated metabolites include cysteine, γ-aminobutyric acid, histidine, hydroxylysine, hydroxyproline, glycine, proline, methionine, ornithine, tryptophan, aspartic acid, argininosuccinic acid, α-aminoadipic acid, and β-aminoisobutyric acid. RF algorithm defined a set of 15 metabolites that constitute the significant features of teratozoospermia. In particular, increased glutamine, asparagine, and decreased cysteine, tryptophan, glycine, and valine were strong predictors of teratozoospemia. The most affected metabolic pathways in teratozoospermic men are the aminoacyl-tRNA, arginine, valine-leucine, and isoleucine biosynthesis. Altered metabolites detected in teratozoospermia were responsible for various roles in sperm functions that classified into four subgroups as follows: related metabolites to antioxidant function, energy production, sperm function, and spermatogenesis. The altered amino acid metabolome identified in this study may be related to the etiology of teratozoospermia, and may provide novel insight into potential biomarkers of male infertility for therapeutic targets.
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Registered trials
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