Evidence map›Paper›PMID 37262927›Full record

ArticleACS applied bio materials2023

Fluorometric Determination of DNA Nanostructure Biostability.

Hannah Talbot, Bharath Raj Madhanagopal, Andrew Hayden, Ken Halvorsen, Arun Richard Chandrasekaran

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS applied bio materials, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Hannah TalbotThe RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.
Bharath Raj MadhanagopalThe RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.ORCID 0000-0003-1043-8754
Andrew HaydenThe RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.
Ken HalvorsenThe RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.ORCID 0000-0002-2578-1339
Arun Richard ChandrasekaranThe RNA Institute, University at Albany, State University of New York, Albany, New York 12203, United States.ORCID 0000-0001-6757-5464
Albany State University · US

Funding

Manipulating nucleic acids: applications in RNA biosensing, single-molecule analysis, and DNA nanotechnologyR35GM124720 · NIGMS · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI Ken A Halvorsen · 2017 to 2026
$4.6M
DNA Nanostructures as siRNA Delivery Vehicles for Alzheimer's TherapyR03AG076599 · NIA · STATE UNIVERSITY OF NEW YORK AT ALBANY · PI CHANDRASEKARAN, ARUN RICHARD · 2022 to 2023
$425k
NIA NIH HHS R03 AG076599NIGMS NIH HHS R35 GM124720
6 · The paper itself

Abstract

The analysis and improvement of DNA nanostructure biostability is one of the keys areas of progress needed in DNA nanotechnology applications. Here, we present a plate-compatible fluorometric assay for measuring DNA nanostructure biostability using the common intercalator ethidium bromide. We demonstrate the assay by testing the biostability of duplex DNA, a double crossover DNA motif, and a DNA origami nanostructure against different nucleases and in fetal bovine serum. This method scales well to measure a large number of samples using a plate reader and can complement existing methods for assessing and developing robust DNA nanostructures.

Indexed as

NanostructuresDeoxyribonucleasesDNAFluorometryDeoxyribonucleasesDNAbiostabilityDNA nanostructuresDNA nanotechnologyDNA origaminuclease degradation

Identifiers

PMID37262927
PMCPMC10999248
OpenAlexW4378975060

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.