Evidence map›Paper›PMID 37261586›Full record

ArticleJournal of assisted reproduction and genetics2023

Trehalose delivered by cold-responsive nanoparticles improves tolerance of cumulus-oocyte complexes to microwave drying.

Pei-Chih Lee, Samantha Stewart, Olga Amelkina, Hannah Sylvester, Xiaoming He, Pierre Comizzoli

Abstract read
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In one paragraph

Article in Journal of assisted reproduction and genetics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Biobanking biodiversity: recent strategies, challenges, and opportunities.The Journal of reproduction and development · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Pei-Chih LeeSmithsonian's National Zoo and Conservation Biology Institute, Washington, D.C., USA.ORCID http://orcid.org/0000-0002-7121-5047
Samantha StewartFischell Department of Bioengineering, University of Maryland, College Park, MD, USA.
Olga AmelkinaSmithsonian's National Zoo and Conservation Biology Institute, Washington, D.C., USA.
Hannah SylvesterSmithsonian's National Zoo and Conservation Biology Institute, Washington, D.C., USA.
Xiaoming HeFischell Department of Bioengineering, University of Maryland, College Park, MD, USA.ORCID http://orcid.org/0000-0003-0125-6086
Pierre ComizzoliSmithsonian's National Zoo and Conservation Biology Institute, Washington, D.C., USA. comizzolip@si.edu.ORCID http://orcid.org/0000-0003-3079-9063

Funding

Drying, Storing, and Reanimating Egg Germinal Vesicles to Preserve FertilityR01OD023139 · OD · SMITHSONIAN INSTITUTION · PI COMIZZOLI, PIERRE · 2017 to 2021
$2.2M
NIH HHS R01 OD023139
6 · The paper itself

Abstract

purposeTrehalose is a non-permeable protectant that is the key to preserve live cells in a dry state for potential storage at ambient temperatures. After intracellular trehalose delivery via cold-responsive nanoparticles (CRNPs), the objective was to characterize the tolerance of cat cumulus-oocyte complexes (COCs) to different levels of microwave-assisted dehydration.

methodsTrehalose was first encapsulated in CRNPs. After exposure to trehalose-laden CRNPs, different water amounts were removed from cat COCs by microwave drying. After each dehydration level, meiotic and developmental competences were evaluated via in vitro maturation, fertilization, and embryo culture. In addition, expressions of critical genes were assessed by quantitative RT-PCR.

resultsCRNPs effectively transported trehalose into COCs within 4 h of co-incubation at 38.5 °C followed by a cold-triggered release at 4 °C for 15 min. Intracellular presence of trehalose enabled the maintenance of developmental competence (formation of blastocysts) as well as normal gene expression levels of HSP70 and DNMT1 at dehydration levels reaching up to 63% of water loss.

conclusionIntracellular trehalose delivery through CRNPs improves dehydration tolerance of COCs, which opens new options for oocyte storage and fertility preservation at ambient temperatures.

Indexed as

In Vitro Oocyte Maturation TechniquesTrehaloseCumulus CellsDehydrationFemaleHumansMicrowavesOocytesTrehaloseCold-responsive nanoparticlesCumulus-oocyte complexFertility preservationMicrowave-assisted dehydrationTrehalose

Identifiers

PMID37261586
PMCPMC10371938

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.