Evidence map›Paper›PMID 37259257›Full record

ArticlePhotochemistry and photobiology

Caged luciferins enable rapid multicomponent bioluminescence imaging.

Mariana X Navarro, Caroline K Brennan, Anna C Love, Jennifer A Prescher

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Photochemistry and photobiology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Engineering luminopsins with improved coupling efficiencies.bioRxiv : the preprint server for biology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mariana X NavarroDepartment of Chemistry, University of California, Irvine, Irvine, California, USA.
Caroline K BrennanDepartment of Chemistry, University of California, Irvine, Irvine, California, USA.
Anna C LoveDepartment of Chemistry, University of California, Irvine, Irvine, California, USA.
Jennifer A PrescherDepartment of Chemistry, University of California, Irvine, Irvine, California, USA.ORCID 0000-0002-9250-4702

Funding

Univ.of Calif., Irvine Cancer Center Support GrantP30CA062203 · NCI · UNIVERSITY OF CALIFORNIA-IRVINE · PI Melanie Funes · 1994 to 2026
$57.9M
purchase of an All-in-One Fluorescence MicroscopeR01GM107630 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI PRESCHER, JENNIFER · 2013 to 2021
$2.6M
New tools for quantitative non-invasive recording of biochemical signalsR01CA279813 · NCI · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI Jennifer Prescher, Nathan Christopher Shaner · 2024 to 2026
$2.0M
NCI NIH HHS P30 CA062203NCI NIH HHS R01 CA279813NIGMS NIH HHS R01 GM107630
6 · The paper itself

Abstract

Bioluminescence is a sensitive technique for imaging biological features over time. Historically, though, the modality has been challenging to employ for multiplexed tracking due to a lack of resolvable luciferase-luciferin pairs. Recent years have seen the development of numerous orthogonal probes for multi-parameter imaging. While successful, generating such tools often requires complex syntheses and lengthy enzyme evolution campaigns. This work showcases an alternative strategy for multiplexed bioluminescence that takes advantage of already-orthogonal caged luciferins and established uncaging enzymes. These probes generate unique bioluminescent signals that can be distinguished via a linear unmixing algorithm. Caged luciferins enabled two- and three-component imaging on the minutes time scale. We further showed that the tools can be used in conjunction with endogenous enzymes for multiplexed studies. Collectively, this approach lowers the barrier to multicomponent bioluminescence imaging and can be readily adopted by the broader community.

Indexed as

LuciferinsLuminescent MeasurementsFirefly LuciferinLuciferasesFirefly LuciferinLuciferasesLuciferinsbioluminescenceimagingluciferaseluciferinsubstrate unmixing

Identifiers

PMID37259257
PMCPMC10687313

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.