Evidence map›Paper›PMID 37258877›Full record

ArticleBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]2023

Stable isotope labeling as a promising tool for rapid drug susceptibility testing in Neisseria gonorrhoeae.

Rajneesh Dadwal, Saikat Paul, Parakriti Gupta, Rakesh Yadav, Seema Sood, A K Ghosh, M R Shivaprakash, Shalini Gainder, Sunil Sethi

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PubMed Full text at PMC Publisher
In one paragraph

Article in Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology], 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.4field-weighted citation impact, top 41% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Rajneesh DadwalDepartment of Medical Microbiology, PGIMER, Chandigarh, 160012, India.
Saikat PaulDepartment of Medical Microbiology, PGIMER, Chandigarh, 160012, India.
Parakriti GuptaDepartment of Medical Microbiology, PGIMER, Chandigarh, 160012, India.
Rakesh YadavDepartment of Medical Microbiology, PGIMER, Chandigarh, 160012, India.
Seema SoodDepartment of Microbiology, AIIMS, New Delhi, India.
A K GhoshDepartment of Medical Microbiology, PGIMER, Chandigarh, 160012, India.
M R ShivaprakashDepartment of Medical Microbiology, PGIMER, Chandigarh, 160012, India.
Shalini GainderDepartment of Obstetrics and Gynaecology, PGIMER, Chandigarh, 160012, India.
Sunil SethiDepartment of Medical Microbiology, PGIMER, Chandigarh, 160012, India. sunilsethi10@gmail.com.
Post Graduate Institute of Medical Education and Research · INAll India Institute of Medical Sciences Bhopal · IN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The world is heading towards an era of intractable and impending untreatable N. gonorrhoeae, thereby underlining the significance of rapid and accurate prediction of drug resistance as an indispensable need of the hour. In the present study, we optimized and evaluated a stable isotope labeling-based approach using the MALDI-TOF MS (Matrix-Assisted Laser Desorption/Ionization-Time of Flight Mass Spectrometry) for rapid and reliable detection of ciprofloxacin and azithromycin resistance in N. gonorrhoeae. All the isolates were cultured under three varied condition setups viz. medium supplemented with normal lysine, heavy lysine (isotope), and heavy lysine along with the antibiotics (ciprofloxacin/azithromycin), respectively. After incubation, spectra were acquired using the MALDI-TOF MS which were further screened for unique patterns (media-specific spectra) to differentiate drug-susceptible and resistant isolates. The results of the stable isotope labeling assay were comparable to the results of phenotypic methods used for susceptibility testing.

Indexed as

Mycobacterium tuberculosisNeisseria gonorrhoeaeAnti-Bacterial AgentsAzithromycinCiprofloxacinCulture Media, ConditionedIsotope LabelingLysineMicrobial Sensitivity TestsSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationAnti-Bacterial AgentsAzithromycinCiprofloxacinCulture Media, ConditionedLysineCiprofloxacinMALDI-TOFResistance detectionSexually transmitted infectionSILAC

Identifiers

PMID37258877
PMCPMC10485193
OpenAlexW4378953249

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.