Evidence map›Paper›PMID 37258768›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2023

Quantitation of Glutamine Synthetase 1 Activity in Drosophila melanogaster.

Teresa Vitali, Maria Antonietta Vanoni, Paola Bellosta

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.5field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Teresa VitaliDipartimento di Bioscienze, Università degli Studi di Milano, Milan, Italy.ORCID https://orcid.org/0000-0002-3435-1736
Maria Antonietta VanoniDipartimento di Bioscienze, Università degli Studi di Milano, Milan, Italy. maria.vanoni@unimi.it.ORCID https://orcid.org/0000-0001-7213-732X
Paola BellostaDepartment of Cellular, Computational and Integrative Biology (CIBIO), University of Trento, Trento, Italy. paola.bellosta@unitn.it.ORCID https://orcid.org/0000-0003-1913-5661
University of Milan · ITUniversity of Trento · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protocols to assay the activity of glutamine synthetase (GS) are presented as they have been used in our laboratory to correlate the expression levels of the gene encoding Drosophila GS1 gene, the GS1 protein levels, and its activity in extracts of larvae and heads from Drosophila melanogaster. The assays are based on the glutamine synthetase-catalyzed formation of γ-glutamylhydroxylamine in the presence of ATP, L-glutamate, and hydroxylamine, in which hydroxylamine substitutes for ammonia in the reaction. Formation of γ-glutamylhydroxylamine is monitored spectrophotometrically in discontinuous assays upon complex formation with FeCl

Indexed as

Drosophila melanogasterGlutamate-Ammonia LigaseAnimalsDrosophilaGlutamic AcidGlutamineLigasesGlutamate-Ammonia LigaseGlutamic AcidGlutamineLigasesActivity assayAutophagyDrosophila melanogasterGlutamine synthetaseHuntington diseaseL-GlutamineL-Glutamine-L-glutamate cycleNeuronal diseases

Identifiers

PMID37258768
OpenAlexW4378805460

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.