ArticleScientific reports2023
Extracellular vesicles secreted by Brugia malayi microfilariae modulate the melanization pathway in the mosquito host.
Article in Scientific reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Host, vector, and parasite dynamics: exploring intrinsic and extrinsic factors shaping tick-borne filarial nematode transmission.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Advancing insect research through cell line transcriptomics.In vitro cellular & developmental biology. Animal · 2026Review
- Extracellular Vesicles in Arthropods: Biogenesis, Functions, Isolation Methods and Applications.Journal of extracellular vesicles · 2025Review
- The immunomodulatory p43 secreted protein of Trichuris whipworm parasites is a lipid carrier that binds signalling lipids and precursors.Scientific reports · 2025Article
- TNF signaling mediates cellular immune function and promotes malaria parasite killing in the mosquito Anopheles gambiae.PLoS pathogens · 2025Article
- Extracellular vesicles from fifth-stage larvalmSystems · 2025Article
- Proteomic analysis unveils host-parasite interactions in Aedes togoi infected with Dirofilaria immitis and Brugia pahangi.PloS one · 2025Article
- Article
- Wolbachia infection in Aedes aegypti does not affect its vectorial capacity for Dirofilaria immitis.Scientific reports · 2024Article
- Immune Reactions of Vector Insects to Parasites and Pathogens.Microorganisms · 2024Review
- Overview of extracellular vesicles in pathogens with special focus on human extracellular protozoan parasites.Memorias do Instituto Oswaldo Cruz · 2024Review
- Identification of biomarker candidates for filarial parasite infections by analysis of extracellular vesicles.Frontiers in parasitology · 2023Article
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Authors and funding
10 authors.
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Abstract
Vector-borne, filarial nematode diseases cause significant disease burdens in humans and domestic animals worldwide. Although there is strong direct evidence of parasite-driven immunomodulation of mammalian host responses, there is less evidence of parasite immunomodulation of the vector host. We have previously reported that all life stages of Brugia malayi, a filarial nematode and causative agent of Lymphatic filariasis, secrete extracellular vesicles (EVs). Here we investigate the immunomodulatory effects of microfilariae-derived EVs on the vector host Aedes aegypti. RNA-seq analysis of an Ae. aegypti cell line treated with B. malayi microfilariae EVs showed differential expression of both mRNAs and miRNAs. AAEL002590, an Ae. aegypti gene encoding a serine protease, was shown to be downregulated when cells were treated with biologically relevant EV concentrations in vitro. Injection of adult female mosquitoes with biologically relevant concentrations of EVs validated these results in vivo, recapitulating the downregulation of AAEL002590 transcript. This gene was predicted to be involved in the mosquito phenoloxidase (PO) cascade leading to the canonical melanization response and correspondingly, both suppression of this gene using RNAi and parasite EV treatment reduced PO activity in vivo. Our data indicate that parasite-derived EVs interfere with critical immune responses in the vector host, including melanization.
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