ArticleFrontiers in veterinary science2023
Development and application of a TaqMan-based real-time PCR method for the detection of the ASFV MGF505-7R gene.
Article in Frontiers in veterinary science, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- Review
- Development of Real-Time and Lateral Flow Dipstick Recombinase Polymerase Amplification Assays for the Rapid Field Diagnosis of MGF-505R Gene-Deleted Mutants of African Swine Fever Virus.Veterinary sciences · 2025Article
- A phosphoprotein gene RT-qPCR assay for detecting all lineages of peste des petits ruminants virus.Frontiers in microbiology · 2025Article
- Assessment of Nine Real-Time PCR Kits for African Swine Fever Virus Approved in Republic of Korea.Viruses · 2024Article
- Development of plate-type and tubular chemiluminescence immunoassay against African swine fever virus p72.Applied microbiology and biotechnology · 2024Article
- Enhanced detection of African swine fever virus in samples with low viral load using digital PCR technology.Heliyon · 2024Article
- Overview of Modern Commercial Kits for Laboratory Diagnosis of African Swine Fever and Swine Influenza A Viruses.Viruses · 2024Review
- A triple protein-based ELISA for differential detection of ASFV antibodies.Frontiers in veterinary science · 2024Article
- Development of rapid nucleic acid testing techniques for common respiratory infectious diseases in the Chinese population.Frontiers in chemistry · 2024Article
Corrections and comments
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
African swine fever virus (ASFV), the etiological agent of African swine fever (ASF), causes deadly hemorrhagic fever in domestic pigs. ASF's high mortality and morbidity have had disastrous effects on the world's swine industry. In recent years, the number of African swine virus strains has increased and presented new challenges for detecting classical ASFV-p72-based viruses. In this study, we observed that the ASFV MGF505-7R gene, a member of the multigene family that can enhance ASFV virulence and pathogenesis, has the potential to be a candidate for vaccine formulations. We also developed a real-time PCR assay based on the ASFV MGF505-7R gene and validated it in multiple aspects. The results indicated that the approach could detect standard plasmids with a sensitivity and a specificity of up to 1 × 10
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