Evidence map›Paper›PMID 37253917›Full record

ArticleMolecular biology reports2023

Screening and validation of salt-stress responsive cg-SSR markers in wheat (Triticum aestivum L.) germplasm of Pakistan.

Mishal Khalid, Rehana Kausar, Armghan Shahzad, Ghulam Muhammad Ali, Sania Begum

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Article in Molecular biology reports, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.2field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Mishal KhalidDepartment of Botany, University of Azad Jammu & Kashmir, Muzaffarabad, 13100, Pakistan.ORCID http://orcid.org/0009-0002-6954-1561
Rehana KausarNational Institute for Genomics and Advanced Biotechnology, National Agriculture Research Center, Park Road, Islamabad, 45500, Pakistan. rehana_ajku@yahoo.com.ORCID http://orcid.org/0000-0002-7131-925X
Armghan ShahzadNational Institute for Genomics and Advanced Biotechnology, National Agriculture Research Center, Park Road, Islamabad, 45500, Pakistan.ORCID http://orcid.org/0000-0002-1387-6445
Ghulam Muhammad AliNational Institute for Genomics and Advanced Biotechnology, National Agriculture Research Center, Park Road, Islamabad, 45500, Pakistan.ORCID http://orcid.org/0000-0002-5206-4167
Sania BegumNational Institute for Genomics and Advanced Biotechnology, National Agriculture Research Center, Park Road, Islamabad, 45500, Pakistan.
Abdus Salam Centre for Physics · PKUniversity of Azad Jammu and Kashmir · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSoil salinity has been affecting wheat production worldwide over past few decades. Evaluation of wheat genotypes for salinity tolerance at germination and vegetative growth level is crucial. Marker assisted selection is a technique used extensively for choosing salt-tolerant genotypes from breeding populations to introduce novel genes. METHODS AND MATERIALS: The current study's main goal was to discover salt-stress resistant genes; genetic divergence and genome-wide connection by using recently designed candidate gene-based simple-sequence-repeat markers (cg-SSRs). The phenotypic connection of morphological features during the germination growth stage i.e., germination period, root length/weight and shoot length/weight, and vegetative growth stages i.e., root length/weight and shoot length/weight were tested in a group of 50 wheat genotypes. Significant difference was observed in germination rate, root length and weight among control and saline treatments.

conclusionTotal 30 SSR markers were utilized to test salinity resistance genes in wheat genotypes. Three (10%) of which were monomorphic, one (3.34%) showed no result, and the other 26 (86%) were polymorphic. Using 30 polymorphic markers discovered total 37 alleles. The polymorphic information content (PIC), quantifies each SSR locus capacity to discriminate between wheat, varied from 0.00 to 0.38 with an average of 0.19. Association analysis revealed that 26 primers were associated with morphological features, 03 with root length and the remaining 23 with germination. Utilizing morphological data, stress tolerance index (STI) was designed concluding that Auqab-2000, Margala-99 and Ufaq showed better resistance against salinity among other wheat genotypes. Cluster analysis demonstrated that wheat genotypes have vast genetic variability.

Indexed as

Plant BreedingTriticumGenotypePakistanSalt ToleranceMarker-assisted selectionSalinitySSRsWheat

Identifiers

PMID37253917
OpenAlexW4378782258

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.