Evidence map›Paper›PMID 37251394›Full record

ArticleFrontiers in immunology2023

The manipulation of cell suspensions from zebrafish intestinal mucosa contributes to understanding enteritis.

Xuyang Zhao, Yuhang Liu, Jiayuan Xie, Lei Zhang, Qingsong Zhu, Lian Su, Cheng Guo, Heng Li, Guangxin Wang, Wanting Zhang and 3 more

Open access · goldAbstract read
In one paragraph

Article in Frontiers in immunology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 6 citations in OpenAlex.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 4 institutions in 1 country.

Xuyang ZhaoInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Yuhang LiuInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Jiayuan XieInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Lei ZhangInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Qingsong ZhuInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Lian SuInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Cheng GuoInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Heng LiInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Guangxin WangInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Wanting ZhangInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Yingyin ChengInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Nan WuInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Xiao-Qin XiaInstitute of Hydrobiology, Chinese Academy of Sciences, Wuhan, China.
Institute of Hydrobiology · CNDalian Ocean University · CNChinese Academy of Sciences · CNUniversity of Chinese Academy of Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background: Although zebrafish are commonly used to study intestinal mucosal immunity, no dedicated procedure for isolating immune cells from zebrafish intestines is currently available. A speedy and simple operating approach for preparing cell suspension from mucosa has been devised to better understanding of intestinal cellular immunity in zebrafish. Methods and results: The mucosal villi were separated away from the muscle layer by repeated blows. The complete deprivation of mucosa was done and evidenced by HE and Conclusion: As a result, the current work created a realistic technique for studying intestinal immune cells in zebrafish. The immune cells acquired may aid in further research and knowledge of intestinal illness at the cellular level.

Indexed as

EnteritisZebrafishAnimalsCytokinesIntestinal MucosaIntestinesCytokinescell suspensionflow cytometryintestineSBMIEtranscriptomezebrafish

Identifiers

PMID37251394
PMCPMC10213505
OpenAlexW4378746659

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.