ArticlePakistan journal of medical sciences
Comparison of single and double centrifugation methods for preparation of Platelet-Rich Plasma (PRP).
Article in Pakistan journal of medical sciences. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 27 citations in OpenAlex.
- A comparative study of the efficacy of single and double centrifuged platelet-rich plasma and placebo (normal saline injection) on pain, ROM, and physical function of patients with knee osteoarthritis: a double-blinded controlled randomized trial with a six-month follow-up.Advances in rheumatology (London, England) · 2026Trial
- Effect of Plasma Gel on Viability, Proliferation and Migration of Dermal Human Fibroblasts In Vitro.Gels (Basel, Switzerland) · 2026Article
- Analysis of platelet rich plasma preparations by routine centrifuge versus two commercially available standardized centrifuges.Pakistan journal of medical sciences · 2026Article
- From Regenerative Mechanisms to Clinical Practice: Current Status, Controversies, and Future Perspectives of Platelet-Rich Plasma in Urology and Sexual Medicine.Journal of clinical medicine · 2026Review
- Experimental esophageal reconstruction in dogs using autologous platelet-rich plasma-augmented acellular amniotic membrane scaffold.Open veterinary journal · 2026Article
- Platelet-rich plasma in cartilage repair: biological mechanisms and clinical perspectives.Frontiers in sports and active living · 2026Review
- Platelet-rich plasma-contained drug delivery systems to treat orthopedic injuries.International journal of pharmaceutics: X · 2025Review
- The Efficiency of Platelet-Rich Plasma (PRP) in Treating Post-Burn and Surgical Scars: A Meta-Analysis Study.Journal of clinical medicine · 2025Review
- Platelet-derived exosomes in situ reprogramming macrophages for rheumatoid arthritis treatment.Cell communication and signaling : CCS · 2025Article
- Mitochondrial transplantation and platelet rich plasma for the treatment of non-alcoholic fatty liver disease.World journal of hepatology · 2025Review
- Effect of Fixed-Angle and Horizontal Rotor Centrifugation on Optimized Platelet-Rich Plasma Preparation.Aesthetic plastic surgery · 2025Article
- Potential Applications of PRP-Enhanced Polybutylene Succinate Graft as Vascular Access for Chemotherapy in Oncological Patients: A Systematic Review.Journal of functional biomaterials · 2025Review
- From Adhesions to Conception: A Case Study on Platelet-Rich Plasma's Role in Gynecologic Recovery.Cureus · 2025Article
- Effect of platelet-rich plasma combined with simvastatin in the treatment of steroid-induced avascular necrosis of the femoral head.Pakistan journal of medical sciences · 2025Article
- Inflammatory Signatures and Biological Markers in Platelet-Rich Plasma Therapy for Hair Regrowth: A Comprehensive Narrative Analysis.Diagnostics (Basel, Switzerland) · 2025Review
- Utilization of Platelet-Rich Plasma in Oral Surgery: A Systematic Review of the Literature.Journal of clinical medicine · 2025Review
- Review
- Review
- Platelet-Rich Plasma Inhibits Breast Cancer Proliferation.Clinical Medicine Insights. Oncology · 2024Article
- The Clinical Efficacy of Platelet-Rich Plasma Injection Therapy versus Different Control Groups for Chronic Low Back Pain: A Network Meta-Analysis of Randomized Controlled Trials.Journal of pain research · 2024Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Objective: To compare the platelet count, platelet concentration/yield, residual Red blood cells (RBCs) and White blood cells (WBCs) counts in platelet-rich plasma (PRP) samples prepared from the single- and the double-centrifugation protocols. Methods: It was a Cross-Sectional study, conducted at the Department of Hematology & Transfusion Medicine, The Children's Hospital and UCHS, Lahore from October 2021 to January 2022 including 50 voluntary, healthy individuals of age 20-45 years of both genders, after taking informed consent. Complete blood count analysis of all participants was done initially by drawing 3ml blood in EDTA vial. From all the participants, 20 ml venous blood sample was taken in syringes containing tri-sodium citrate and then shifted to harvest tubes. Group-I comprised of PRP samples prepared by single- centrifugation method. While Group-II samples were prepared by Double-centrifugation method consisting of soft and hard spin. The platelet, RBC and WBC counts in prepared PRP samples were determined by using automated SYSMEX XP-100 hematology analyzer. Platelet yield or Platelet concentration (%) was calculated for samples using formula. The data analysis was done using SPSS version 23. Results: The mean PRP platelet count in Group-I was 594.6±157.4×10 Conclusions: The double centrifugation protocol resulted in higher platelet quantity and yield with less contamination by red and white blood cells than did the single centrifugation protocol for PRP preparation. So, double centrifugation method is beneficial in preparation of autologous as well as allogenic PRP.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.