Evidence map›Paper›PMID 37250442›Full record

ArticleEcology and evolution2023

Development of short-target primers for species identification in biological studies of Carnivora.

Huiwen Liu, Dan Wang, Chenglin Zhang, Tianchun Pu, Lijuan Xiong, Fuwen Wei, Yibo Hu

Abstract read
In one paragraph

Article in Ecology and evolution, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Huiwen LiuCAS Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology, Chinese Academy of Sciences Beijing China.
Dan WangCAS Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology, Chinese Academy of Sciences Beijing China.
Chenglin ZhangBeijing Zoo Beijing China.
Tianchun PuBeijing Zoo Beijing China.
Lijuan XiongSchool of Life Sciences Guizhou Normal University Guiyang China.
Fuwen WeiCAS Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology, Chinese Academy of Sciences Beijing China.ORCID https://orcid.org/0000-0002-5233-1288
Yibo HuCAS Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology, Chinese Academy of Sciences Beijing China.ORCID https://orcid.org/0000-0003-3409-3164

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Noninvasive genetic sampling greatly facilitates studies on the genetics, ecology, and conservation of threatened species. Species identification is often a prerequisite for noninvasive sampling-based biological studies. Due to the low quantity and quality of genomic DNA from noninvasive samples, high-performance short-target PCR primers are necessary for DNA barcoding applications. The order Carnivora is characterized by an elusive habit and threatened status. In this study, we developed three pairs of short-target primers for identifying Carnivora species. The COI279 primer pair was suitable for samples with better DNA quality. The COI157a and COI157b primer pairs performed well for noninvasive samples and reduced the interference of nuclear mitochondrial pseudogenes (numts). COI157a could effectively identify samples from Felidae, Canidae, Viverridae, and Hyaenidae, while COI157b could be applied to samples from Ursidae, Ailuridae, Mustelidae, Procyonidae, and Herpestidae. These short-target primers will facilitate noninvasive biological studies and efforts to conserve Carnivora species.

Indexed as

CarnivoraCOInoninvasive samplespecies identification

Identifiers

PMID37250442
PMCPMC10212699

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.