ArticleMethods in molecular biology (Clifton, N.J.)2023
Transfection of Barley Leaf Protoplasts with a Fluorescently Tagged Fungal Effector for In Planta Localization Studies in Barley.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
The isolation and transfection of protoplasts from plant leaves have been routinely used for transient expression and functional studies in model plants. However, current approaches to characterize pathogen effector molecules in a cereal host are inefficient and technically challenging. In this chapter, we describe a protocol to isolate and transfect barley mesophyll protoplasts with a fluorescently tagged fungal effector of the barley smut pathogen Ustilago hordei. Tagging of a fungal effector with a fluorescent protein and tracking its localization in cells of its natural host provides insight into its putative in planta localization and helps to narrow down the location of putative host interactors.
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