ArticleMethods in molecular biology (Clifton, N.J.)2023
Live-Cell Visualization of Early Stages of Root Colonization by the Vascular Wilt Pathogen Fusarium oxysporum.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
5 citing papers in PubMed, 6 citations in OpenAlex.
- Phase-contrast microtomography unveils mechanisms of root colonization by a vascular fungal pathogen.Nature communications · 2026Article
- Mating-Type Loci Modulate Pathogenicity and Non-Sexual Development Through Autocrine Pheromone Signalling in the Asexual Fungus Fusarium oxysporum.Molecular plant pathology · 2026Article
- The cardamom-Fusarium pathosystem: current knowledge and future directions.World journal of microbiology & biotechnology · 2025Review
- Profiling Metabolites with Antifungal Activities from Endophytic Plant-Beneficial Strains ofMolecules (Basel, Switzerland) · 2024Article
- An improved method to study Phytophthora cinnamomi Rands zoospores interactions with host.BMC plant biology · 2024Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fungal phytopathogens induce a variety of pathogenicity symptoms on their hosts. The soilborne vascular wilt pathogen Fusarium oxysporum infects roots of more than 150 different crop species. Initial colonization stages are asymptomatic, likely representing a biotrophic phase of infection, followed by a necrotrophic switch after vascular colonization which results in blockage of the plant xylem and killing of the host. Live-cell microscopy techniques have been successfully employed to study interaction events during fungal colonization of root tissues. This technique is widely used to track fungal development during disease progression. Here, we describe a well-established protocol for generation and screening of fluorescently tagged F. oxysporum transformants, as well as for live-cell imaging of the early colonization stages of F. oxysporum on tomato (Solanum lycopersicum) seedlings. The presented experimental design and techniques involved are also applicable to other root infecting fungi.
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Registered trials
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