ArticleMethods in molecular biology (Clifton, N.J.)2023
Single-Molecule Fluorescence In Situ Hybridization for Spatial Detection of mRNAs in Sections of Mammalian Testes.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed, 2 citations in OpenAlex.
- HiPlex RNAscope™ for In Situ Analysis of Gene Expression During Murine Thymocyte Differentiation.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Spatial Transcriptomic Analyses of Spermatogenesis.Methods in molecular biology (Clifton, N.J.) · 2025Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Single-molecule fluorescence in situ hybridization (smFISH) enables the detection and localization of individual mRNAs in tissue sections with single-molecule resolution while preserving spatial context, and thus, is a useful tool for examining gene expression in biological systems. In particular, the growing reliance on single-cell RNA sequencing (scRNA-seq) to explore cellular heterogeneity has reinvigorated this approach as a validation tool to spatially re-map mRNA expression patterns described in isolated cells to their parent tissue. While use of antibody-based methods, such as indirect immunofluorescence (IIF), remain popular as validation strategies, smFISH often affords superior specificity and maintains congruency with scRNA-seq. Here, we present a detailed protocol that combines multiplexed smFISH using the RNAscope approach with IIF to co-visualize mRNAs and proteins within sections of mouse testes. We provide step-by-step guidelines from testis preparation through visualization that enables mapping of combinations of up to four mRNA/protein targets in frozen sections on the RNAscope platform.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.