Evidence map›Paper›PMID 37249689›Full record

ArticleFunctional & integrative genomics2023

Interaction of C/EBPβ with SMAD2 and SMAD4 genes induces the formation of lipid droplets in bovine myoblasts.

Dawei Wei, Le Zhang, Sayed Haidar Abbas Raza, Jiupan Zhang, Zhao Juan, Hadba Al-Amrah, Waleed Al Abdulmonem, Yousef Mesfer Alharbi, Guijie Zhang, Xiaojun Liang

Abstract read
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In one paragraph

Article in Functional & integrative genomics, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.5field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 3 citations in OpenAlex.

  1. CDK5RAP3 Regulates Testosterone Production in Mouse Leydig Cells.International journal of molecular sciences · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 7 institutions in 2 countries.

Dawei Wei *College of Animal Science and Technology, Ningxia University, Yinchuan, 750021, China.
Le Zhang *Institute of Physical Education, Yan'an University, Yan'an, 716000, China.
Sayed Haidar Abbas RazaGuangdong Provincial Key Laboratory of Food Quality and Safety/Nation-Local Joint Engineering Research Center for Machining and Safety of Livestock and Poultry Products, South China Agricultural University, Guangzhou, 510642, China.
Jiupan ZhangInstitute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, 750021, China.
Zhao JuanCollege of Animal Science and Technology, South China Agricultural University, Guangzhou, 510642, China.
Hadba Al-AmrahDepartment of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia.
Waleed Al AbdulmonemDepartment of Pathology, College of Medicine, Qassim University, P.O. Box 6655, Buraidah, 51452, Kingdom of Saudi Arabia.
Yousef Mesfer AlharbiDepartment of Veterinary Medicine, College of Agriculture and Veterinary Medicine, Qassim University, Buraydah, 51452, Saudi Arabia.
Guijie ZhangCollege of Animal Science and Technology, Ningxia University, Yinchuan, 750021, China. guijiezhang@nxu.edu.cn.
Xiaojun LiangInstitute of Animal Science, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan, 750021, China. nxnkycx@163.com.
Ningxia Academy of Agriculture and Forestry Sciences · CNNingxia University · CNQassim University · SAKing Abdulaziz University · SANorthwest A&F University · CNSouth China Agricultural University · CNYan'an University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

As a key component of Transforming growth factor-β (TGF-β) pathway, Smad2 has many crucial roles in a variety of cellular processes, but it cannot bind DNA without complex formation with Smad4. In the present study, the molecular mechanism in the progress of myogenesis underlying transcriptional regulation of SMAD2 and SMAD4 had been clarified. The result showed the inhibition between SMAD2 and SMAD4, which promotes and inhibits bovine myoblast differentiation, respectively. Further, the characterization of promoter region of SMAD2 and SMAD4 was analyzed, and identified C/EBPβ directly bound to the core region of both SMAD2 and SMAD4 genes promoter and stimulated the transcriptional activity. However, C/EBPβ has lower expression in myoblasts which plays vital function in the transcriptional networks controlling adipogenesis, while the overexpression of C/EBPβ gene in myoblasts significantly increased SMAD2 and SMAD4 gene expression, induced the formation of lipid droplet in bovine myoblasts, and promoted the expression of adipogenesis-specific genes. Collectively, our results showed that C/EBPβ may play an important role in the trans-differentiation and dynamic equilibrium of myoblasts into adipocyte cells via promoting an increase in SMAD2 and SMAD4 gene levels. These results will provide an important basis for further understanding of the TGFβ pathway and C/EBPβ gene during myogenic differentiation.

Indexed as

CCAAT-Enhancer-Binding Protein-betaLipid DropletsAnimalsCattleCell DifferentiationMyoblastsSignal TransductionTransforming Growth Factor betaCCAAT-Enhancer-Binding Protein-betaTransforming Growth Factor betaC/EBPβMyoblastSMAD2SMAD4Transcriptional regulation

Identifiers

PMID37249689
OpenAlexW4378746219

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.