Evidence map›Paper›PMID 37248392›Full record

ReviewNature protocols2023

Spatial- and Fourier-domain ptychography for high-throughput bio-imaging.

Shaowei Jiang, Pengming Song, Tianbo Wang, Liming Yang, Ruihai Wang, Chengfei Guo, Bin Feng, Andrew Maiden, Guoan Zheng

Open access · greenAbstract readReview
In one paragraph

Review in Nature protocols, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed
30.1field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 84 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Article
  6. Multiscale aperture synthesis imager.Nature communications · 2025
    Article
  7. Review
  8. Article
  9. Article
  10. Uncertainty-aware Fourier ptychography.Light, science & applications · 2025
    Article
  11. Review
  12. High-Fidelity Computational Microscopy via Feature-Domain Phase Retrieval.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025
    Article
  13. Article
  14. Ptychography at all wavelengths.Nature reviews. Methods primers · 2025
    Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. On the use of deep learning for phase recovery.Light, science & applications · 2024
    Review
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 3 countries.

Shaowei Jiang *Department of Biomedical Engineering, University of Connecticut, Storrs, USA.
Pengming Song *Department of Biomedical Engineering, University of Connecticut, Storrs, USA.
Tianbo Wang *Department of Biomedical Engineering, University of Connecticut, Storrs, USA.
Liming YangDepartment of Biomedical Engineering, University of Connecticut, Storrs, USA.
Ruihai WangDepartment of Biomedical Engineering, University of Connecticut, Storrs, USA.
Chengfei GuoDepartment of Biomedical Engineering, University of Connecticut, Storrs, USA.
Bin FengDepartment of Biomedical Engineering, University of Connecticut, Storrs, USA.
Andrew MaidenDepartment of Electronic and Electrical Engineering, University of Sheffield, Sheffield, UK.
Guoan ZhengDepartment of Biomedical Engineering, University of Connecticut, Storrs, USA. guoan.zheng@uconn.edu.ORCID 0000-0002-4268-2690
University of Connecticut · USDiamond Light Source · GB

Funding

Determining the topology and molecular profiles of nociceptive DRG neurons innervating distal colon and rectumU01NS113873 · NINDS · UNIVERSITY OF CONNECTICUT STORRS · PI FENG, BIN · 2019 to 2021
$2.0M
NINDS NIH HHS U01 NS113873
6 · The paper itself

Abstract

First envisioned for determining crystalline structures, ptychography has become a useful imaging tool for microscopists. However, ptychography remains underused by biomedical researchers due to its limited resolution and throughput in the visible light regime. Recent developments of spatial- and Fourier-domain ptychography have successfully addressed these issues and now offer the potential for high-resolution, high-throughput optical imaging with minimal hardware modifications to existing microscopy setups, often providing an excellent trade-off between resolution and field of view inherent to conventional imaging systems, giving biomedical researchers the best of both worlds. Here, we provide extensive information to enable the implementation of ptychography by biomedical researchers in the visible light regime. We first discuss the intrinsic connections between spatial-domain coded ptychography and Fourier ptychography. A step-by-step guide then provides the user instructions for developing both systems with practical examples. In the spatial-domain implementation, we explain how a large-scale, high-performance blood-cell lens can be made at negligible expense. In the Fourier-domain implementation, we explain how adding a low-cost light source to a regular microscope can improve the resolution beyond the limit of the objective lens. The turnkey operation of these setups is suitable for use by professional research laboratories, as well as citizen scientists. Users with basic experience in optics and programming can build the setups within a week. The do-it-yourself nature of the setups also allows these procedures to be implemented in laboratory courses related to Fourier optics, biomedical instrumentation, digital image processing, robotics and capstone projects.

Indexed as

Image Processing, Computer-AssistedMicroscopyOptical Imaging

Identifiers

PMID37248392
PMCPMC12034321
OpenAlexW4378674289

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.