ArticleNature communications2023
Quantitative structured illumination microscopy via a physical model-based background filtering algorithm reveals actin dynamics.
Article in Nature communications, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Review
- The multifaceted significance of phosphoinositides in endocytic trafficking.FEBS letters · 2026Review
- Advanced Imaging for Live-Cell Spatiotemporal Monitoring: Technologies and Applications.Research (Washington, D.C.) · 2026Review
- Visualizing intraorganellar ultrastructures, dynamics, and interactions with open-access background-free Lock-in-SIM.Nature communications · 2025Article
- Cellular optical imaging techniques: a dynamic advancing frontier.Science China. Life sciences · 2025Review
- Time-deterministic cryo-optical microscopy.Light, science & applications · 2025Article
- Optical tweeze-sectioning microscopy for 3D imaging and manipulation of suspended cells.Science advances · 2025Article
- Dark-based optical sectioning assists background removal in fluorescence microscopy.Nature methods · 2025Article
- Bright and photostable yellow fluorescent proteins for extended imaging.Nature communications · 2025Article
- Computational Super-Resolution: An Odyssey in Harnessing Priors to Enhance Optical Microscopy Resolution.Analytical chemistry · 2025Review
- Fast reconstruction and optical-sectioning three-dimensional structured illumination microscopy.Innovation (Cambridge (Mass.)) · 2025Article
- Super-resolution imaging of the neuronal cytoskeleton.Npj imaging · 2024Review
- Nanoscale insights into hematology: super-resolved imaging on blood cell structure, function, and pathology.Journal of nanobiotechnology · 2024Review
- Ultra-high spatio-temporal resolution imaging with parallel acquisition-readout structured illumination microscopy (PAR-SIM).Light, science & applications · 2024Article
- Single-molecule tracking reveals the functional allocation, in vivo interactions, and spatial organization of universal transcription factor NusG.Molecular cell · 2024Article
- Live-cell imaging in the deep learning era.Current opinion in cell biology · 2023Review
- Projective oblique plane structured illumination microscopy.Npj imaging · 2023Article
- Super-Resolution Imaging of Neuronal Structures with Structured Illumination Microscopy.Bioengineering (Basel, Switzerland) · 2023Article
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Authors and funding
14 authors.
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Abstract
Despite the prevalence of superresolution (SR) microscopy, quantitative live-cell SR imaging that maintains the completeness of delicate structures and the linearity of fluorescence signals remains an uncharted territory. Structured illumination microscopy (SIM) is the ideal tool for live-cell SR imaging. However, it suffers from an out-of-focus background that leads to reconstruction artifacts. Previous post hoc background suppression methods are prone to human bias, fail at densely labeled structures, and are nonlinear. Here, we propose a physical model-based Background Filtering method for living cell SR imaging combined with the 2D-SIM reconstruction procedure (BF-SIM). BF-SIM helps preserve intricate and weak structures down to sub-70 nm resolution while maintaining signal linearity, which allows for the discovery of dynamic actin structures that, to the best of our knowledge, have not been previously monitored.
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