ArticleMethods in molecular biology (Clifton, N.J.)2023
Working with Immortalized Hepatic Stellate Cell Lines.
Article in Methods in molecular biology (Clifton, N.J.), 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- Article
- <p>Beyond hepatic stellate cell heterogeneity: Resolving fibrosis, restoring regeneration (Review)</p>.International journal of molecular medicine · 2026Review
- Article
- Comprehensive Genetic and Molecular Characterization Confirms Hepatic Stellate Cell Origin of the Immortal Col-GFP HSC Line.International journal of molecular sciences · 2025Article
- Misidentified cell lines: failures of peer review, varying journal responses to misidentification inquiries, and strategies for safeguarding biomedical research.Research integrity and peer review · 2025Article
- Functional Liver Cell-Based Platforms in Biomedical Research.Pharmacology research & perspectives · 2025Review
- Furin inhibits HSCs activation and ameliorates liver fibrosis by regulating PTEN-L/PINK1/parkin mediated mitophagy in mouse.FASEB bioAdvances · 2025Article
- Unlocking Potential: A Comprehensive Overview of Cell Culture Banks and Their Impact on Biomedical Research.Cells · 2024Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors.
Funding
Abstract
Hepatic stellate cells (HSCs) are the major cellular source of extracellular matrix production in the liver. Therefore, this cell population has received considerable attention in studies investigating fundamental features of hepatic fibrosis. However, the limited supply and ever-increasing demand for these cells, combined with the additional tightening of formal standards in animal welfare policy, make working with these primary cells increasingly difficult. Moreover, researchers working in biomedical research are challenged to implement the 3R principle of "replacement," "reduction," and "refinement" in their work. This principle, originally proposed in 1959 by William M. S. Russell and Rex L. Burch, is now widely endorsed by legislators and regulatory bodies in many countries as a roadmap to tackle the ethical dilemma associated with animal experimentation. As such, working with immortalized HSC lines is a good alternative to limit the number of animals and their suffering in biomedical research. This article summarizes issues that need to be considered when working with established HSC cell lines and provides general guidelines for the maintenance and storage of HSC lines from mouse, rat, and humans.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.