Evidence map›Paper›PMID 37246820›Full record

ArticleACS synthetic biology2023

Interlaboratory Reproducibility in Growth and Reporter Expression in the Cyanobacterium

Maurice Mager, Hugo Pineda Hernandez, Fabian Brandenburg, Luis López-Maury, Alistair J McCormick, Dennis J Nürnberg, Tim Orthwein, David A Russo, Angelo Joshua Victoria, Xiaoran Wang and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in ACS synthetic biology, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Maurice MagerInstitute for Synthetic Microbiology, Heinrich Heine University Duesseldorf, Universitaetsstrasse 1, 40225 Duesseldorf, Germany.
Hugo Pineda HernandezMolecular Microbial Physiology Group, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, Amsterdam 1098 XH, The Netherlands.
Fabian BrandenburgHelmholtz Centre for Environmental Research (UFZ), Permoserstrasse 15, 04318 Leipzig, Germany.
Luis López-MauryInstituto de Bioquímica Vegetal y Fotosíntesis, University of Seville - CSIC, Américo Vespucio 49, 41092 Sevilla, Spain.
Alistair J McCormickInstitute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, 1.04 Daniel Rutherford Building, King's Buildings, EH9 3BF Edinburgh, U.K.ORCID 0000-0002-7255-872X
Dennis J NürnbergDepartment of Physics, Experimental Biophysics, Freie University Berlin, Arnimallee 14, 14195 Berlin, Germany.
Tim OrthweinInterfaculty Institute of Microbiology and Infection Medicine, University of Tuebingen, Auf der Morgenstelle 28, 72076 Tübingen, Germany.
David A RussoInstitute for Inorganic and Analytical Chemistry, Bioorganic Analytics, Friedrich Schiller University Jena, Lessingstrasse 8, 07743 Jena, Germany.
Angelo Joshua VictoriaInstitute of Molecular Plant Sciences, School of Biological Sciences, University of Edinburgh, 1.04 Daniel Rutherford Building, King's Buildings, EH9 3BF Edinburgh, U.K.
Xiaoran WangDepartment of Physics, Experimental Biophysics, Freie University Berlin, Arnimallee 14, 14195 Berlin, Germany.
Julie A Z ZedlerMatthias Schleiden Institute for Genetics, Bioinformatics and Molecular Botany, Synthetic Biology of Photosynthetic Organisms, Friedrich Schiller University Jena, Dornburgerstrasse 159, 07743 Jena, Germany.ORCID 0000-0002-0462-8810
Filipe Branco Dos SantosMolecular Microbial Physiology Group, Swammerdam Institute for Life Sciences, Faculty of Science, University of Amsterdam, Science Park 904, Amsterdam 1098 XH, The Netherlands.ORCID 0000-0002-4268-8080
Nicolas M SchmellingInstitute for Synthetic Microbiology, Heinrich Heine University Duesseldorf, Universitaetsstrasse 1, 40225 Duesseldorf, Germany.

Funding

Biotechnology and Biological Sciences Research Council BB/S020128/1
6 · The paper itself

Abstract

In recent years, a plethora of new synthetic biology tools for use in cyanobacteria have been published; however, their reported characterizations often cannot be reproduced, greatly limiting the comparability of results and hindering their applicability. In this interlaboratory study, the reproducibility of a standard microbiological experiment for the cyanobacterial model organism

Indexed as

SynechocystisBiomassGenes, ReporterPromoter Regions, GeneticReproducibility of Resultscyanobacteriainterlabpromoterreproducibility

Identifiers

PMID37246820
PMCPMC10278186

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.