Evidence map›Paper›PMID 37244593›Full record

ArticleThe ocular surface2023

Novel discovery of a lymphatic bridge connecting Schlemm's canal to limbal and conjunctival lymphatic pathway.

Yujia Yang, Meng Shi, Guangyu Li, Lejun Shen, Lu Chen

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The ocular surface, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 10 citations in OpenAlex.

  1. Article
  2. Review
  3. Ontogenesis of the Mouse Ocular Surface Lymphatic Vascular Network.Investigative ophthalmology & visual science · 2023
    Article
  4. International journal of ophthalmology · 2023
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Yujia YangVision Science Graduate Program, University of California, Berkeley, USA; Center for Eye Disease and Development, Herbert Wertheim School of Optometry and Vision Science, University of California, Berkeley, USA.
Meng ShiVision Science Graduate Program, University of California, Berkeley, USA; Center for Eye Disease and Development, Herbert Wertheim School of Optometry and Vision Science, University of California, Berkeley, USA.
Guangyu LiVision Science Graduate Program, University of California, Berkeley, USA; Center for Eye Disease and Development, Herbert Wertheim School of Optometry and Vision Science, University of California, Berkeley, USA.
Lejun ShenVision Science Graduate Program, University of California, Berkeley, USA; Center for Eye Disease and Development, Herbert Wertheim School of Optometry and Vision Science, University of California, Berkeley, USA.
Lu ChenVision Science Graduate Program, University of California, Berkeley, USA; Center for Eye Disease and Development, Herbert Wertheim School of Optometry and Vision Science, University of California, Berkeley, USA; Francis I. Proctor Foundation for Research in Ophthalmology, University of California San Francisco, USA. Electronic address: chenlu@berkeley.edu.
Berkeley Eye Center · USUniversity of California, San Francisco · US

Funding

Molecular Regulation of Lymphangiogenesis in Corneal AlloimmunityR01EY017392 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHEN, LU · 2007 to 2020
$5.7M
Novel characterization and therapeutic intervention of Schlemm's canal for glaucoma treatmentR01EY028995 · NEI · UNIVERSITY OF CALIFORNIA BERKELEY · PI CHEN, LU · 2018 to 2021
$1.6M
NEI NIH HHS R01 EY017392NEI NIH HHS R01 EY028995
6 · The paper itself

Abstract

purposeSchlemm's canal (SC) is a critical structure regulating aqueous humor (AH) drainage and intraocular pressure (IOP). It is known that in the conventional outflow pathway, AH flows from SC to episcleral veins. We recently reported a high-resolution three-dimensional (3D) imaging technology for intact eyeballs, SC and ocular surface. Using this advanced technology, we herein report the discovery of a new structure, termed lymphatic bridge, that directly connects SC to the limbal and conjunctival lymphatic pathway. Further investigation on this novel outflow pathway may provide new mechanisms and therapeutic approaches for glaucoma.

methodsAs reported previously, intact eyeballs were harvested from Prox-1-GFP (green fluorescent protein) mice and processed by a tissue clearing technique with CLARITY. Samples were immunolabeled with specific antibodies for CD31 (pan-endothelial marker) and LYVE-1 (lymphatic vessel endothelial hyaluronan receptor-1) and imaged by light-sheet fluorescent microscopy. The limbal areas were examined to locate connecting channels between SC and limbal and conjunctival lymphatic vessels. Moreover, in vivo anterior chamber dye injection was performed with Texas Red dextran for functional analysis on AH outflow.

resultsA novel lymphatic bridge structure that expressed both Prox-1 and LYVE-1 was discovered between the SC and limbal lymphatic vessels connected with conjunctival lymphatic pathway. Results from the anterior chamber dye injection also confirmed AH drainage into the conjunctival lymphatic outflow pathway.

conclusionsThis study provides the first evidence on the direct connection between SC and conjunctival lymphatic pathway. This new pathway is distinctive from the traditional episcleral vein pathway and merits further investigation.

Indexed as

Lymphatic VesselsTrabecular MeshworkAnimalsAqueous HumorMiceSchlemm's CanalScleraAqueous humor drainage pathwayConjunctivaLimbusLymphatic vesselSchlemm's canal

Identifiers

PMID37244593
PMCPMC10567112
OpenAlexW4378190274

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.