Evidence map›Paper›PMID 37243884›Full record

ArticleOrigins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life2023

Nonenzymatic Template-Directed Primer Extension Using 2'-3' Cyclic Nucleotides Under Wet-Dry Cycles.

Shikha Dagar, Susovan Sarkar, Sudha Rajamani

Abstract read
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In one paragraph

Article in Origins of life and evolution of the biosphere : the journal of the International Society for the Study of the Origin of Life, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Shikha DagarDepartment of Biology, Indian Institute of Science Education and Research, Pune, 411008, India.
Susovan SarkarDepartment of Biology, Indian Institute of Science Education and Research, Pune, 411008, India.
Sudha RajamaniDepartment of Biology, Indian Institute of Science Education and Research, Pune, 411008, India. srajamani@iiserpune.ac.in.

Funding

Department of Biotechnology, Ministry of Science and Technology, India BT/PR19201/BRB/10/1532/2016Science and Engineering Research Board CRG/2021/001851
6 · The paper itself

Abstract

RNA World Hypothesis is centred around the idea of a period in the early history of life's origin, wherein nonenzymatic oligomerization and replication of RNA resulted in functional ribozymes. Previous studies in this endeavour have demonstrated template-directed primer extension using chemically modified nucleotides and primers. Nonetheless, similar studies that used non-activated nucleotides led to the formation of RNA only with abasic sites. In this study, we report template-directed primer extension with prebiotically relevant cyclic nucleotides, under dehydration-rehydration (DH-RH) cycles occurring at high temperature (90 °C) and alkaline conditions (pH 8). 2'-3' cyclic nucleoside monophosphates (cNMP) resulted in primer extension, while 3'-5' cNMP failed to do so. Intact extension of up to two nucleotide additions was observed with both canonical hydroxy-terminated (OH-primer) and activated amino-terminated (NH

Indexed as

NucleotidesNucleotides, CyclicRNANucleotidesNucleotides, CyclicRNACyclic nucleotidesDehydration-rehydration cyclesPrebiotic chemistryStrand separationTemplate-directed primer extension

Identifiers

PMID37243884

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.