Evidence map›Paper›PMID 37241650›Full record

ArticleMicromachines2023

High-Efficiency Single-Cell Containment Microdevices Based on Fluid Control.

Daiki Tanaka, Junichi Ishihara, Hiroki Takahashi, Masashi Kobayashi, Aya Miyazaki, Satsuki Kajiya, Risa Fujita, Naoki Maekawa, Yuriko Yamazaki, Akiko Takaya and 4 more

Abstract read
In one paragraph

Article in Micromachines, 2023. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Daiki TanakaResearch Organization for Nano & Life Innovation, Waseda University, 513 Waseda Tsurumakicho, Shinjuku-ku, Tokyo 162-0041, Japan.ORCID 0000-0003-1900-4848
Junichi IshiharaMedical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-0856, Japan.
Hiroki TakahashiMedical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-0856, Japan.ORCID 0000-0001-5627-1035
Masashi KobayashiSchool of Fundamental Science and Engineering, Waseda University, 3-4-1 Okubo, Shin-juku-ku, Tokyo 169-8555, Japan.
Aya MiyazakiSchool of Fundamental Science and Engineering, Waseda University, 3-4-1 Okubo, Shin-juku-ku, Tokyo 169-8555, Japan.
Satsuki KajiyaSchool of Fundamental Science and Engineering, Waseda University, 3-4-1 Okubo, Shin-juku-ku, Tokyo 169-8555, Japan.
Risa FujitaResearch Organization for Nano & Life Innovation, Waseda University, 513 Waseda Tsurumakicho, Shinjuku-ku, Tokyo 162-0041, Japan.
Naoki MaekawaDepartment of Natural Products Chemistry, Graduate School of Pharmaceutical Sciences, Chiba University, Chiba 260-8675, Japan.
Yuriko YamazakiDepartment of Dermatology, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.ORCID 0000-0002-4026-8306
Akiko TakayaMedical Mycology Research Center, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-0856, Japan.
Yuumi NakamuraDepartment of Dermatology, Osaka University Graduate School of Medicine, Osaka 565-0871, Japan.
Masahiro FuruyaGraduate School of Advanced Science and Engineering, Waseda University, 3-4-1 Okubo, Shinjuku-ku, Tokyo 169-8555, Japan.ORCID 0000-0002-1221-2874
Tetsushi SekiguchiResearch Organization for Nano & Life Innovation, Waseda University, 513 Waseda Tsurumakicho, Shinjuku-ku, Tokyo 162-0041, Japan.
Shuichi ShojiSchool of Fundamental Science and Engineering, Waseda University, 3-4-1 Okubo, Shin-juku-ku, Tokyo 169-8555, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we developed a comb-shaped microfluidic device that can efficiently trap and culture a single cell (bacterium). Conventional culture devices have difficulty in trapping a single bacterium and often use a centrifuge to push the bacterium into the channel. The device developed in this study can store bacteria in almost all growth channels using the flowing fluid. In addition, chemical replacement can be performed in a few seconds, making this device suitable for culture experiments with resistant bacteria. The storage efficiency of microbeads that mimic bacteria was significantly improved from 0.2% to 84%. We used simulations to investigate the pressure loss in the growth channel. The pressure in the growth channel of the conventional device was more than 1400 PaG, whereas that of the new device was less than 400 PaG. Our microfluidic device was easily fabricated by a soft microelectromechanical systems method. The device was highly versatile and can be applied to various bacteria, such as

Indexed as

cell culturefluid controlmicrofluidic devicessingle cell

Identifiers

PMID37241650
PMCPMC10222914

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.